4ewj: Difference between revisions

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-[[Image:4ewj.jpg|left|200px]]
+==structure of the enloase from Streptococcus suis serotype 2==
+<StructureSection load='4ewj' size='340' side='right' caption='[[4ewj]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[4ewj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_suis Streptococcus suis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EWJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EWJ FirstGlance]. <br>
+</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1w6t|1w6t]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">eno, eno2, enolase, SSUST3_1361 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1307 Streptococcus suis])</td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ewj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ewj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ewj RCSB], [http://www.ebi.ac.uk/pdbsum/4ewj PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Enolase is a conserved cytoplasmic metalloenzyme existing universally in both eukaryotic and prokaryotic cells. The enzyme can also locate on the cell surface and bind to plasminogen, via which contributing to the mucosal surface localization of the bacterial pathogens and assisting the invasion into the host cells. The functions of the eukaryotic enzymes on the cell surface expression (including T cells, B cells, neutrophils, monocytoes, neuronal cells and epithelial cells) are not known. Streptococcus suis serotype 2 (S. suis 2, SS2) is an important zoonotic pathogen which has recently caused two large-scale outbreaks in southern China with severe streptococcal toxic shock syndrome (STSS) never seen before in human sufferers. We recently identified the SS2 enolase as an important protective antigen which could protect mice from fatal S.suis 2 infection. In this study, a 2.4-angstrom structure of the SS2 enolase is solved, revealing an octameric arrangement in the crystal. We further demonstrated that the enzyme exists exclusively as an octamer in solution via a sedimentation assay. These results indicate that the octamer is the biological unit of SS2 enolase at least in vitro and most likely in vivo as well. This is, to our knowledge, the first comprehensive characterization of the SS2 enolase octamer both structurally and biophysically, and the second octamer enolase structure in addition to that of Streptococcus pneumoniae. We also investigated the plasminogen binding property of the SS2 enzyme.
-{{STRUCTURE_4ewj|  PDB=4ewj  |  SCENE=  }}
+An octamer of enolase from Streptococcus suis.,Lu Q, Lu H, Qi J, Lu G, Gao GF Protein Cell. 2012 Oct;3(10):769-80. doi: 10.1007/s13238-012-2040-7. Epub 2012, Oct 11. PMID:23055041<ref>PMID:23055041</ref>
-===structure of the enloase from Streptococcus suis serotype 2===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_23055041}}
+==See Also==
+*[[Enolase|Enolase]]
-==About this Structure==
+== References ==
-[[4ewj]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_suis Streptococcus suis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EWJ OCA].
+<references/>
+__TOC__
+</StructureSection>
 [[Category: Phosphopyruvate hydratase]]
 [[Category: Streptococcus suis]]
-[[Category: Gao, G F.]]
+[[Category: Gao, G F]]
-[[Category: Lu, G.]]
+[[Category: Lu, G]]
-[[Category: Lu, H.]]
+[[Category: Lu, H]]
-[[Category: Lu, Q.]]
+[[Category: Lu, Q]]
-[[Category: Qi, J.]]
+[[Category: Qi, J]]
 [[Category: Glycolytic pathway involved enzyme]]
 [[Category: Lyase]]
 [[Category: Plasminogen binding]]
 [[Category: Two-domain enzyme]]