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[[ | ==Crystal structure of rhesus TRIM5alpha PRY/SPRY domain== | ||
<StructureSection load='4b3n' size='340' side='right' caption='[[4b3n]], [[Resolution|resolution]] 3.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4b3n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Macaca_mulatta Macaca mulatta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4B3N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4B3N FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAL:MALTOSE'>MAL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1anf|1anf]], [[1dmb|1dmb]], [[1ez9|1ez9]], [[1ezo|1ezo]], [[1ezp|1ezp]], [[1fqa|1fqa]], [[1fqb|1fqb]], [[1fqc|1fqc]], [[1fqd|1fqd]], [[1iud|1iud]], [[1jvx|1jvx]], [[1jvy|1jvy]], [[1jw4|1jw4]], [[1jw5|1jw5]], [[1lax|1lax]], [[1lls|1lls]], [[1mdp|1mdp]], [[1mdq|1mdq]], [[1mpb|1mpb]], [[1mpc|1mpc]], [[1mpd|1mpd]], [[1n3w|1n3w]], [[1n3x|1n3x]], [[1nl5|1nl5]], [[1nmu|1nmu]], [[1omp|1omp]], [[1peb|1peb]], [[1r6z|1r6z]], [[1svx|1svx]], [[1t0k|1t0k]], [[1y4c|1y4c]], [[1ytv|1ytv]], [[1ziu|1ziu]], [[1zjl|1zjl]], [[1zkb|1zkb]], [[1zmg|1zmg]], [[2d21|2d21]], [[2h25|2h25]], [[2vgq|2vgq]], [[2xz3|2xz3]], [[3mbp|3mbp]], [[4auf|4auf]], [[4aug|4aug]], [[4auh|4auh]], [[4mbp|4mbp]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4b3n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4b3n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4b3n RCSB], [http://www.ebi.ac.uk/pdbsum/4b3n PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Tripartite motif protein isoform 5 alpha (TRIM5alpha) is a potent antiviral protein that restricts infection by HIV-1 and other retroviruses. TRIM5alpha recognizes the lattice of the retrovirus capsid through its B30.2 (PRY/SPRY) domain in a species-specific manner. Upon binding, TRIM5alpha induces premature disassembly of the viral capsid and activates the downstream innate immune response. We have determined the crystal structure of the rhesus TRIM5alpha PRY/SPRY domain that reveals essential features for capsid binding. Combined cryo-electron microscopy and biochemical data show that the monomeric rhesus TRIM5alpha PRY/SPRY, but not the human TRIM5alpha PRY/SPRY, can bind to HIV-1 capsid protein assemblies without causing disruption of the capsid. This suggests that the PRY/SPRY domain alone constitutes an important pattern-sensing component of TRIM5alpha that is capable of interacting with viral capsids of different curvatures. Our results provide molecular insights into the mechanisms of TRIM5alpha-mediated retroviral restriction. | |||
Structural insight into HIV-1 capsid recognition by rhesus TRIM5alpha.,Yang H, Ji X, Zhao G, Ning J, Zhao Q, Aiken C, Gronenborn AM, Zhang P, Xiong Y Proc Natl Acad Sci U S A. 2012 Nov 6;109(45):18372-7. doi:, 10.1073/pnas.1210903109. Epub 2012 Oct 22. PMID:23091002<ref>PMID:23091002</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Macaca mulatta]] | [[Category: Macaca mulatta]] | ||
[[Category: Ji, X | [[Category: Ji, X]] | ||
[[Category: Xiong, Y | [[Category: Xiong, Y]] | ||
[[Category: Yang, H | [[Category: Yang, H]] | ||
[[Category: Zhao, Q | [[Category: Zhao, Q]] | ||
[[Category: Sugar binding protein-ligase chimera]] | [[Category: Sugar binding protein-ligase chimera]] | ||
[[Category: Sugar binding protein-ligase complex]] | [[Category: Sugar binding protein-ligase complex]] | ||
Revision as of 20:05, 9 December 2014
Crystal structure of rhesus TRIM5alpha PRY/SPRY domainCrystal structure of rhesus TRIM5alpha PRY/SPRY domain
Structural highlights
Publication Abstract from PubMedTripartite motif protein isoform 5 alpha (TRIM5alpha) is a potent antiviral protein that restricts infection by HIV-1 and other retroviruses. TRIM5alpha recognizes the lattice of the retrovirus capsid through its B30.2 (PRY/SPRY) domain in a species-specific manner. Upon binding, TRIM5alpha induces premature disassembly of the viral capsid and activates the downstream innate immune response. We have determined the crystal structure of the rhesus TRIM5alpha PRY/SPRY domain that reveals essential features for capsid binding. Combined cryo-electron microscopy and biochemical data show that the monomeric rhesus TRIM5alpha PRY/SPRY, but not the human TRIM5alpha PRY/SPRY, can bind to HIV-1 capsid protein assemblies without causing disruption of the capsid. This suggests that the PRY/SPRY domain alone constitutes an important pattern-sensing component of TRIM5alpha that is capable of interacting with viral capsids of different curvatures. Our results provide molecular insights into the mechanisms of TRIM5alpha-mediated retroviral restriction. Structural insight into HIV-1 capsid recognition by rhesus TRIM5alpha.,Yang H, Ji X, Zhao G, Ning J, Zhao Q, Aiken C, Gronenborn AM, Zhang P, Xiong Y Proc Natl Acad Sci U S A. 2012 Nov 6;109(45):18372-7. doi:, 10.1073/pnas.1210903109. Epub 2012 Oct 22. PMID:23091002[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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