3u43: Difference between revisions

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-[[Image:3u43.png|left|200px]]
+==Crystal structure of the colicin E2 DNase-Im2 complex==
+<StructureSection load='3u43' size='340' side='right' caption='[[3u43]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3u43]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U43 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U43 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">imm, ceiB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), col, ceaB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u43 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u43 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u43 RCSB], [http://www.ebi.ac.uk/pdbsum/3u43 PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+How proteins achieve high-affinity binding to a specific protein partner while simultaneously excluding all others is a major biological problem that has important implications for protein design. We report the crystal structure of the ultra-high-affinity protein-protein complex between the endonuclease domain of colicin E2 and its cognate immunity (Im) protein, Im2 (K(d) approximately 10(-)(15) M), which, by comparison to previous structural and biophysical data, provides unprecedented insight into how high affinity and selectivity are achieved in this model family of protein complexes. Our study pinpoints the role of structured water molecules in conjoining hotspot residues that govern stability with residues that control selectivity. A key finding is that a single residue, which in a noncognate context massively destabilizes the complex through frustration, does not participate in specificity directly but rather acts as an organizing center for a multitude of specificity interactions across the interface, many of which are water mediated.
-{{STRUCTURE_3u43|  PDB=3u43  |  SCENE=  }}
+Structure of the Ultra-High-Affinity Colicin E2 DNase-Im2 Complex.,Wojdyla JA, Fleishman SJ, Baker D, Kleanthous C J Mol Biol. 2012 Mar 16;417(1-2):79-94. Epub 2012 Jan 27. PMID:22306467<ref>PMID:22306467</ref>
-===Crystal structure of the colicin E2 DNase-Im2 complex===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_22306467}}
-==About this Structure==
-[[3u43]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U43 OCA].
 ==See Also==
+*[[Colicin|Colicin]]
 *[[Colicin Immunity Protein|Colicin Immunity Protein]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:022306467</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Escherichia coli]]
-[[Category: Kleanthous, C.]]
+[[Category: Kleanthous, C]]
-[[Category: Wojdyla, J A.]]
+[[Category: Wojdyla, J A]]
 [[Category: Dnase]]
 [[Category: High affinity]]
 [[Category: Protein binding]]
 [[Category: Protein-protein complex]]