1nt9: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1nt9.gif|left|200px]] | [[Image:1nt9.gif|left|200px]] | ||
'''Complete 12-subunit RNA polymerase II''' | {{Structure | ||
|PDB= 1nt9 |SIZE=350|CAPTION= <scene name='initialview01'>1nt9</scene>, resolution 4.20Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] | |||
|GENE= | |||
}} | |||
'''Complete 12-subunit RNA polymerase II''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1NT9 is a [ | 1NT9 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NT9 OCA]. | ||
==Reference== | ==Reference== | ||
Architecture of initiation-competent 12-subunit RNA polymerase II., Armache KJ, Kettenberger H, Cramer P, Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):6964-8. Epub 2003 May 13. PMID:[http:// | Architecture of initiation-competent 12-subunit RNA polymerase II., Armache KJ, Kettenberger H, Cramer P, Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):6964-8. Epub 2003 May 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12746495 12746495] | ||
[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| Line 19: | Line 28: | ||
[[Category: dna-dependent rna polymerase; cellular rna polymerase; multisubunit complex; transcription; gene expression; mrna; messenger rna synthesis]] | [[Category: dna-dependent rna polymerase; cellular rna polymerase; multisubunit complex; transcription; gene expression; mrna; messenger rna synthesis]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:01:21 2008'' | ||
Revision as of 14:01, 20 March 2008
| |||||||
| , resolution 4.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Activity: | DNA-directed RNA polymerase, with EC number 2.7.7.6 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Complete 12-subunit RNA polymerase II
OverviewOverview
RNA polymerase (Pol) II consists of a 10-polypeptide catalytic core and the two-subunit Rpb4/7 complex that is required for transcription initiation. Previous structures of the Pol II core revealed a "clamp," which binds the DNA template strand via three "switch regions," and a flexible "linker" to the C-terminal repeat domain (CTD). Here we derived a model of the complete Pol II by fitting structures of the core and Rpb4/7 to a 4.2-A crystallographic electron density map. Rpb4/7 protrudes from the polymerase "upstream face," on which initiation factors assemble for promoter DNA loading. Rpb7 forms a wedge between the clamp and the linker, restricting the clamp to a closed position. The wedge allosterically prevents entry of the promoter DNA duplex into the active center cleft and induces in two switch regions a conformation poised for template-strand binding. Interaction of Rpb4/7 with the linker explains Rpb4-mediated recruitment of the CTD phosphatase to the CTD during Pol II recycling. The core-Rpb7 interaction and some functions of Rpb4/7 are apparently conserved in all eukaryotic and archaeal RNA polymerases but not in the bacterial enzyme.
About this StructureAbout this Structure
1NT9 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Architecture of initiation-competent 12-subunit RNA polymerase II., Armache KJ, Kettenberger H, Cramer P, Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):6964-8. Epub 2003 May 13. PMID:12746495
Page seeded by OCA on Thu Mar 20 13:01:21 2008