1ngx: Difference between revisions

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Revision as of 13:56, 20 March 2008

File:1ngx.gif

, resolution 1.80Å

Ligands:

Coordinates:

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Chimeric Germline Fab 7g12 with jeffamine fragment bound

OverviewOverview

The crystal structure of the Michaelis complex between the Fab fragment of ferrochelatase antibody 7G12 and its substrate mesoporphyrin has been solved to 2.6-A resolution. The antibody-bound mesoporphyrin clearly adopts a nonplanar conformation and reveals that the antibody catalyzes the porphyrin metallation reaction by straining/distorting the bound substrate toward the transition-state configuration. The crystal structures of the Fab fragment of the germ-line precursor antibody to 7G12 and its complex with the hapten N-methylmesoporphyrin have also been solved. A comparison of these structures with the corresponding structures of the affinity-matured antibody 7G12 reveals the molecular mechanism by which the immune system evolves binding energy to catalyze this reaction.

About this StructureAbout this Structure

1NGX is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.

ReferenceReference

Structural evidence for substrate strain in antibody catalysis., Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG, Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:12552112 [[Category: ]]

Page seeded by OCA on Thu Mar 20 12:56:51 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1ngx.gif|left|200px]]<br /><applet load="1ngx" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ngx.gif|left|200px]]
-caption="1ngx, resolution 1.80&Aring;" />
-'''Chimeric Germline Fab 7g12 with jeffamine fragment bound'''<br />
+ {{Structure
+|PDB= 1ngx |SIZE=350|CAPTION= <scene name='initialview01'>1ngx</scene>, resolution 1.80&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=JEF:O-(O-(2-AMINOPROPYL)-O'-(2-METHOXYETHYL)POLYPROPYLENE GLYCOL 500)'>JEF</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''Chimeric Germline Fab 7g12 with jeffamine fragment bound'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-NGX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=JEF:'>JEF</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGX OCA].
+NGX is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGX OCA].
 ==Reference==
-Structural evidence for substrate strain in antibody catalysis., Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG, Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12552112 12552112]
+Structural evidence for substrate strain in antibody catalysis., Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG, Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12552112 12552112]
 [[Category: ]]
 [[Category: Protein complex]]
@@ Line 23: / Line 32: @@
 [[Category: immunoglobulin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:05:56 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:56:51 2008''