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[[ | ==RNA Polymerase II Initiation Complex with a 5-nt 3'-deoxy RNA soaked with GTP== | ||
<StructureSection load='3s1r' size='340' side='right' caption='[[3s1r]], [[Resolution|resolution]] 3.20Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3s1r]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3S1R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3S1R FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3s1m|3s1m]], [[3rzo|3rzo]], [[3s1n|3s1n]], [[3s1q|3s1q]], [[3s2d|3s2d]], [[3s2h|3s2h]], [[3rzd|3rzd]], [[3s14|3s14]], [[3s15|3s15]], [[3s16|3s16]], [[3s17|3s17]]</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_RNA_polymerase DNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.6 2.7.7.6] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3s1r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3s1r OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3s1r RCSB], [http://www.ebi.ac.uk/pdbsum/3s1r PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The initiation of transcription by RNA polymerase II is a multistage process. X-ray crystal structures of transcription complexes containing short RNAs reveal three structural states: one with 2- and 3-nucleotide RNAs, in which only the 3'-end of the RNA is detectable; a second state with 4- and 5-nucleotide RNAs, with an RNA-DNA hybrid in a grossly distorted conformation; and a third state with RNAs of 6 nucleotides and longer, essentially the same as a stable elongating complex. The transition from the first to the second state correlates with a markedly reduced frequency of abortive initiation. The transition from the second to the third state correlates with partial "bubble collapse" and promoter escape. Polymerase structure is permissive for abortive initiation, thereby setting a lower limit on polymerase-promoter complex lifetime and allowing the dissociation of nonspecific complexes. Abortive initiation may be viewed as promoter proofreading, and the structural transitions as checkpoints for promoter control. | |||
Initiation complex structure and promoter proofreading.,Liu X, Bushnell DA, Silva DA, Huang X, Kornberg RD Science. 2011 Jul 29;333(6042):633-7. PMID:21798951<ref>PMID:21798951</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[RNA polymerase|RNA polymerase]] | *[[RNA polymerase|RNA polymerase]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: DNA-directed RNA polymerase]] | [[Category: DNA-directed RNA polymerase]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
[[Category: Bushnell, D A | [[Category: Bushnell, D A]] | ||
[[Category: Huang, X | [[Category: Huang, X]] | ||
[[Category: Kornberg, R D | [[Category: Kornberg, R D]] | ||
[[Category: Liu, X | [[Category: Liu, X]] | ||
[[Category: Silva, D A | [[Category: Silva, D A]] | ||
[[Category: Initiation complex]] | [[Category: Initiation complex]] | ||
[[Category: Rna polymerase ii]] | [[Category: Rna polymerase ii]] | ||
[[Category: Transcription-rna-dna complex]] | [[Category: Transcription-rna-dna complex]] | ||
Revision as of 15:48, 9 December 2014
RNA Polymerase II Initiation Complex with a 5-nt 3'-deoxy RNA soaked with GTPRNA Polymerase II Initiation Complex with a 5-nt 3'-deoxy RNA soaked with GTP
Structural highlights
Publication Abstract from PubMedThe initiation of transcription by RNA polymerase II is a multistage process. X-ray crystal structures of transcription complexes containing short RNAs reveal three structural states: one with 2- and 3-nucleotide RNAs, in which only the 3'-end of the RNA is detectable; a second state with 4- and 5-nucleotide RNAs, with an RNA-DNA hybrid in a grossly distorted conformation; and a third state with RNAs of 6 nucleotides and longer, essentially the same as a stable elongating complex. The transition from the first to the second state correlates with a markedly reduced frequency of abortive initiation. The transition from the second to the third state correlates with partial "bubble collapse" and promoter escape. Polymerase structure is permissive for abortive initiation, thereby setting a lower limit on polymerase-promoter complex lifetime and allowing the dissociation of nonspecific complexes. Abortive initiation may be viewed as promoter proofreading, and the structural transitions as checkpoints for promoter control. Initiation complex structure and promoter proofreading.,Liu X, Bushnell DA, Silva DA, Huang X, Kornberg RD Science. 2011 Jul 29;333(6042):633-7. PMID:21798951[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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