3qx8: Difference between revisions

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-[[Image:3qx8.png|left|200px]]
+==Crystal structure of MID domain from hAGO2 in complex with m7GpppG==
+<StructureSection load='3qx8' size='340' side='right' caption='[[3qx8]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3qx8]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QX8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QX8 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3luc|3luc]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EIF2C2, AGO2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qx8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qx8 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qx8 RCSB], [http://www.ebi.ac.uk/pdbsum/3qx8 PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+In RNA silencing, microRNA (miRNA)-mediated translational repression occurs through mechanisms that do not invoke messenger-RNA (mRNA) target cleavage by Argonaute proteins. The nature of these mechanisms is unclear, but several recent studies have proposed that a direct interaction between the mRNA-cap and the middle (MID) domain of Argonautes is involved. Here, we present crystallographic and NMR data demonstrating that cap analogues do not bind significantly to the isolated MID domain of human Argonaute 2 (hAGO2) and are found in the miRNA 5'-nucleotide binding site in an implausible binding mode. Additionally, in vitro pull-down experiments with full-length hAGO2 indicate that the interaction with cap analogues is nonspecific.
-{{STRUCTURE_3qx8|  PDB=3qx8  |  SCENE=  }}
+Structural analysis of 5'-mRNA-cap interactions with the human AGO2 MID domain.,Frank F, Fabian MR, Stepinski J, Jemielity J, Darzynkiewicz E, Sonenberg N, Nagar B EMBO Rep. 2011 Apr 8. PMID:21475248<ref>PMID:21475248</ref>
-===Crystal structure of MID domain from hAGO2 in complex with m7GpppG===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_21475248}}
-==About this Structure==
-[[3qx8]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QX8 OCA].
 ==See Also==
+*[[Argonaute|Argonaute]]
 *[[Eukaryotic initiation factor|Eukaryotic initiation factor]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:021475248</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Homo sapiens]]
-[[Category: Darzynkiewicz, E.]]
+[[Category: Darzynkiewicz, E]]
-[[Category: Fabian, M R.]]
+[[Category: Fabian, M R]]
-[[Category: Frank, F.]]
+[[Category: Frank, F]]
-[[Category: Jemielity, J.]]
+[[Category: Jemielity, J]]
-[[Category: Nagar, B.]]
+[[Category: Nagar, B]]
-[[Category: Sonenberg, N.]]
+[[Category: Sonenberg, N]]
-[[Category: Stepinski, J.]]
+[[Category: Stepinski, J]]
 [[Category: Rna binding]]
 [[Category: Rna binding protein]]
 [[Category: Rossmann-like folde]]
 [[Category: Small rna]]