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[[ | ==A Tandem SH2 Domain in Transcription Elongation Factor Spt6 Binds the Phosphorylated RNA Polymerase II C-terminal Repeat Domain(CTD)== | ||
<StructureSection load='3pjp' size='340' side='right' caption='[[3pjp]], [[Resolution|resolution]] 1.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3pjp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_glabrata Candida glabrata]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3or8 3or8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PJP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PJP FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CAGL0L04774g, SPT6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5478 Candida glabrata])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pjp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pjp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pjp RCSB], [http://www.ebi.ac.uk/pdbsum/3pjp PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Spt6 is an essential transcription elongation factor and histone chaperone that binds the C-terminal repeat domain (CTD) of RNA polymerase (Pol) II. We show here that Spt6 contains a tandem SH2 domain with a novel structure and CTD-binding mode. The tandem SH2 domain binds to a serine 2-phosphorylated CTD peptide in vitro, whereas its N-terminal SH2 subdomain, which we previously characterized, does not. CTD binding requires a positively charged crevice in the C-terminal SH2 subdomain, which lacks the canonical phospho-binding pocket of SH2 domains and had previously escaped detection. The tandem SH2 domain is apparently required for transcription elongation in vivo, as its deletion in cells is lethal in the presence of 6-azauracil. | |||
A tandem SH2 domain in transcription elongation factor Spt6 binds the phosphorylated RNA polymerase II CTD.,Sun M, Lariviere L, Dengl S, Mayer A, Cramer P J Biol Chem. 2010 Oct 6. PMID:20926372<ref>PMID:20926372</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Elongation factor|Elongation factor]] | *[[Elongation factor|Elongation factor]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: Candida glabrata]] | [[Category: Candida glabrata]] | ||
[[Category: Cramer, P | [[Category: Cramer, P]] | ||
[[Category: Dengl, S | [[Category: Dengl, S]] | ||
[[Category: Lariviere, L | [[Category: Lariviere, L]] | ||
[[Category: Mayer, A | [[Category: Mayer, A]] | ||
[[Category: Sun, M | [[Category: Sun, M]] | ||
[[Category: Ctd binding]] | [[Category: Ctd binding]] | ||
[[Category: Nucleus]] | [[Category: Nucleus]] | ||
Revision as of 14:53, 9 December 2014
A Tandem SH2 Domain in Transcription Elongation Factor Spt6 Binds the Phosphorylated RNA Polymerase II C-terminal Repeat Domain(CTD)A Tandem SH2 Domain in Transcription Elongation Factor Spt6 Binds the Phosphorylated RNA Polymerase II C-terminal Repeat Domain(CTD)
Structural highlights
Publication Abstract from PubMedSpt6 is an essential transcription elongation factor and histone chaperone that binds the C-terminal repeat domain (CTD) of RNA polymerase (Pol) II. We show here that Spt6 contains a tandem SH2 domain with a novel structure and CTD-binding mode. The tandem SH2 domain binds to a serine 2-phosphorylated CTD peptide in vitro, whereas its N-terminal SH2 subdomain, which we previously characterized, does not. CTD binding requires a positively charged crevice in the C-terminal SH2 subdomain, which lacks the canonical phospho-binding pocket of SH2 domains and had previously escaped detection. The tandem SH2 domain is apparently required for transcription elongation in vivo, as its deletion in cells is lethal in the presence of 6-azauracil. A tandem SH2 domain in transcription elongation factor Spt6 binds the phosphorylated RNA polymerase II CTD.,Sun M, Lariviere L, Dengl S, Mayer A, Cramer P J Biol Chem. 2010 Oct 6. PMID:20926372[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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