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[[ | ==Crystal Structures of Multidrug-Resistant Clinical Isolate 769 HIV-1 Protease Variants== | ||
<StructureSection load='3pj6' size='340' side='right' caption='[[3pj6]], [[Resolution|resolution]] 2.25Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3pj6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human_immunodeficiency_virus_1 Human immunodeficiency virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PJ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PJ6 FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3oq7|3oq7]], [[3oqa|3oqa]], [[3oqd|3oqd]], [[1tw7|1tw7]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pol ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 Human immunodeficiency virus 1])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pj6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pj6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pj6 RCSB], [http://www.ebi.ac.uk/pdbsum/3pj6 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The flexible flaps and the 80s loops (Pro79-Ile84) of HIV-1 protease are crucial in inhibitor binding. Previously, it was reported that the crystal structure of multidrug-resistant 769 (MDR769) HIV-1 protease shows a wide-open conformation of the flaps owing to conformational rigidity acquired by the accumulation of mutations. In the current study, the effect of mutations on the conformation of the 80s loop of MDR769 HIV-1 protease variants is reported. Alternate conformations of Pro81 (proline switch) with a root-mean-square deviation of 3-4.8 A in the C(alpha) atoms of the I10V mutant and a side chain with a `flipped-out' conformation in the A82F mutant cause distortion in the S1/S1' binding pockets that affects inhibitor binding. The A82S and A82T mutants show local changes in the electrostatics of inhibitor binding owing to the mutation from nonpolar to polar residues. In summary, the crystallographic studies of four variants of MDR769 HIV-1 protease presented in this article provide new insights towards understanding the drug-resistance mechanism as well as a basis for design of future protease inhibitors with enhanced potency. | |||
Contribution of the 80s loop of HIV-1 protease to the multidrug-resistance mechanism: crystallographic study of MDR769 HIV-1 protease variants.,Yedidi RS, Proteasa G, Martinez JL, Vickrey JF, Martin PD, Wawrzak Z, Liu Z, Kovari IA, Kovari LC Acta Crystallogr D Biol Crystallogr. 2011 Jun;67(Pt 6):524-32. Epub 2011, May 17. PMID:21636892<ref>PMID:21636892</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[Virus protease|Virus protease]] | *[[Virus protease|Virus protease]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: Human immunodeficiency virus 1]] | [[Category: Human immunodeficiency virus 1]] | ||
[[Category: Kovari, L C | [[Category: Kovari, L C]] | ||
[[Category: Martin, P D | [[Category: Martin, P D]] | ||
[[Category: Martinez-Cajas, J L | [[Category: Martinez-Cajas, J L]] | ||
[[Category: Proteasa, G | [[Category: Proteasa, G]] | ||
[[Category: Vickrey, J F | [[Category: Vickrey, J F]] | ||
[[Category: Wawrzak, Z | [[Category: Wawrzak, Z]] | ||
[[Category: Yedidi, R S | [[Category: Yedidi, R S]] | ||
[[Category: Alternate conformations of pro81]] | [[Category: Alternate conformations of pro81]] | ||
[[Category: Expanded active site cavity]] | [[Category: Expanded active site cavity]] | ||
Revision as of 14:53, 9 December 2014
Crystal Structures of Multidrug-Resistant Clinical Isolate 769 HIV-1 Protease VariantsCrystal Structures of Multidrug-Resistant Clinical Isolate 769 HIV-1 Protease Variants
Structural highlights
Publication Abstract from PubMedThe flexible flaps and the 80s loops (Pro79-Ile84) of HIV-1 protease are crucial in inhibitor binding. Previously, it was reported that the crystal structure of multidrug-resistant 769 (MDR769) HIV-1 protease shows a wide-open conformation of the flaps owing to conformational rigidity acquired by the accumulation of mutations. In the current study, the effect of mutations on the conformation of the 80s loop of MDR769 HIV-1 protease variants is reported. Alternate conformations of Pro81 (proline switch) with a root-mean-square deviation of 3-4.8 A in the C(alpha) atoms of the I10V mutant and a side chain with a `flipped-out' conformation in the A82F mutant cause distortion in the S1/S1' binding pockets that affects inhibitor binding. The A82S and A82T mutants show local changes in the electrostatics of inhibitor binding owing to the mutation from nonpolar to polar residues. In summary, the crystallographic studies of four variants of MDR769 HIV-1 protease presented in this article provide new insights towards understanding the drug-resistance mechanism as well as a basis for design of future protease inhibitors with enhanced potency. Contribution of the 80s loop of HIV-1 protease to the multidrug-resistance mechanism: crystallographic study of MDR769 HIV-1 protease variants.,Yedidi RS, Proteasa G, Martinez JL, Vickrey JF, Martin PD, Wawrzak Z, Liu Z, Kovari IA, Kovari LC Acta Crystallogr D Biol Crystallogr. 2011 Jun;67(Pt 6):524-32. Epub 2011, May 17. PMID:21636892[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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