1nbu: Difference between revisions
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[[Image:1nbu.gif|left|200px]] | [[Image:1nbu.gif|left|200px]] | ||
'''7,8-Dihydroneopterin Aldolase Complexed with Product From Mycobacterium Tuberculosis''' | {{Structure | ||
|PDB= 1nbu |SIZE=350|CAPTION= <scene name='initialview01'>1nbu</scene>, resolution 1.60Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=PH2:2-AMINO-6-HYDROXYMETHYL-7,8-DIHYDRO-3H-PTERIDIN-4-ONE'>PH2</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Dihydroneopterin_aldolase Dihydroneopterin aldolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.25 4.1.2.25] | |||
|GENE= FOLB OR RV3607C OR MT3712.1 OR MTCY07H7B.15 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 Mycobacterium tuberculosis]) | |||
}} | |||
'''7,8-Dihydroneopterin Aldolase Complexed with Product From Mycobacterium Tuberculosis''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1NBU is a [ | 1NBU is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NBU OCA]. | ||
==Reference== | ==Reference== | ||
Regulation by oligomerization in a mycobacterial folate biosynthetic enzyme., Goulding CW, Apostol MI, Sawaya MR, Phillips M, Parseghian A, Eisenberg D, J Mol Biol. 2005 May 27;349(1):61-72. Epub 2005 Apr 2. PMID:[http:// | Regulation by oligomerization in a mycobacterial folate biosynthetic enzyme., Goulding CW, Apostol MI, Sawaya MR, Phillips M, Parseghian A, Eisenberg D, J Mol Biol. 2005 May 27;349(1):61-72. Epub 2005 Apr 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15876368 15876368] | ||
[[Category: Dihydroneopterin aldolase]] | [[Category: Dihydroneopterin aldolase]] | ||
[[Category: Mycobacterium tuberculosis]] | [[Category: Mycobacterium tuberculosis]] | ||
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[[Category: protein structure initiative]] | [[Category: protein structure initiative]] | ||
[[Category: psi]] | [[Category: psi]] | ||
[[Category: structural | [[Category: structural genomic]] | ||
[[Category: tb structural genomics consortium]] | [[Category: tb structural genomics consortium]] | ||
[[Category: tbsgc]] | [[Category: tbsgc]] | ||
[[Category: two alpha | [[Category: two alpha helice]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:54:48 2008'' | ||
Revision as of 13:54, 20 March 2008
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| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | FOLB OR RV3607C OR MT3712.1 OR MTCY07H7B.15 (Mycobacterium tuberculosis) | ||||||
| Activity: | Dihydroneopterin aldolase, with EC number 4.1.2.25 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
7,8-Dihydroneopterin Aldolase Complexed with Product From Mycobacterium Tuberculosis
OverviewOverview
Folate derivatives are essential cofactors in the biosynthesis of purines, pyrimidines and amino acids across all forms of life. Mammals uptake folate from their diets, whereas most bacteria must synthesize folate de novo. Therefore, the enzymes in the folate biosynthetic pathway are attractive drug targets against bacterial pathogens such as Mycobacterium tuberculosis, the cause of the world's most deadly infectious disease, tuberculosis (TB). M.tuberculosis 7,8-dihydroneopterin aldolase (Mtb FolB, DHNA) is the second enzyme in the folate biosynthetic pathway, which catalyzes the conversion of 7,8-dihydroneopterin to 6-hydroxymethyl-7,8-dihydropterin and glycoaldehyde. The 1.6A X-ray crystal structure of Mtb FolB complexed with its product, 6-hydroxymethyl-7,8-dihydropterin, reveals an octameric assembly similar to that seen in crystal structures of other FolB homologs. However, the 2.5A crystal structure of unliganded Mtb FolB reveals a novel tetrameric oligomerization state, with only partially formed active sites. A substrate induced conformational change appears to be necessary to convert the inactive tetramer to the active octamer. Ultracentrifugation confirmed that in solution unliganded Mtb FolB is mainly tetrameric and upon addition of substrate FolB is predominantly octameric. Kinetic analysis of substrate binding gives a Hill coefficient of 2.0, indicating positive cooperativity. We hypothesize that Mtb FolB displays cooperativity in substrate binding to regulate the cellular concentration of 7,8-dihydroneopterin, so that it may function not only as a precursor to folate but also as an antioxidant for the survival of M.tuberculosis against host defenses.
About this StructureAbout this Structure
1NBU is a Protein complex structure of sequences from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.
ReferenceReference
Regulation by oligomerization in a mycobacterial folate biosynthetic enzyme., Goulding CW, Apostol MI, Sawaya MR, Phillips M, Parseghian A, Eisenberg D, J Mol Biol. 2005 May 27;349(1):61-72. Epub 2005 Apr 2. PMID:15876368
Page seeded by OCA on Thu Mar 20 12:54:48 2008
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Dihydroneopterin aldolase
- Mycobacterium tuberculosis
- Protein complex
- Apostol, M I.
- Eisenberg, D.
- Goulding, C W.
- Parseghian, A.
- Phillips, M.
- Sawaya, M R.
- TBSGC, TB Structural Genomics Consortium.
- PH2
- Anti-parallel
- Beta-sheet
- Protein structure initiative
- Psi
- Structural genomic
- Tb structural genomics consortium
- Tbsgc
- Two alpha helice