3rlg: Difference between revisions

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-[[Image:3rlg.png|left|200px]]
+==Crystal structure of Loxosceles intermedia phospholipase D isoform 1 H12A mutant==
+<StructureSection load='3rlg' size='340' side='right' caption='[[3rlg]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3rlg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Loxosceles_intermedia Loxosceles intermedia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RLG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RLG FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rlh|3rlh]]</td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Sphingomyelin_phosphodiesterase_D Sphingomyelin phosphodiesterase D], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.41 3.1.4.41] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rlg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rlg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rlg RCSB], [http://www.ebi.ac.uk/pdbsum/3rlg PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Phospholipases D are the major dermonecrotic component of Loxosceles venom and catalyze the hydrolysis of phospholipids, resulting in the formation of lipid mediators such as ceramide-1-phosphate and lysophosphatidic acid which can induce pathological and biological responses. Phospholipases D can be classified into two classes depending on their catalytic efficiency and the presence of an additional disulfide bridge. In this work, both wild-type and H12A-mutant forms of the class II phospholipase D from L. intermedia venom were crystallized. Wild-type and H12A-mutant crystals were grown under very similar conditions using PEG 200 as a precipitant and belonged to space group P12(1)1, with unit-cell parameters a = 50.1, b = 49.5, c = 56.5 A, beta = 105.9 degrees . Wild-type and H12A-mutant crystals diffracted to maximum resolutions of 1.95 and 1.60 A, respectively.
-{{STRUCTURE_3rlg|  PDB=3rlg  |  SCENE=  }}
+Crystallization and preliminary X-ray diffraction analysis of a class II phospholipase D from Loxosceles intermedia venom.,Ullah A, de Giuseppe PO, Murakami MT, Trevisan-Silva D, Wille AC, Chaves-Moreira D, Gremski LH, da Silveira RB, Sennf-Ribeiro A, Chaim OM, Veiga SS, Arni RK Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Feb 1;67(Pt, 2):234-6. Epub 2011 Jan 22. PMID:21301094<ref>PMID:21301094</ref>
-===Crystal structure of Loxosceles intermedia phospholipase D isoform 1 H12A mutant===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_21301094}}
-==About this Structure==
-[[3rlg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Loxosceles_intermedia Loxosceles intermedia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RLG OCA].
 ==See Also==
 *[[Sphingomyelinase|Sphingomyelinase]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:021301094</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Loxosceles intermedia]]
 [[Category: Sphingomyelin phosphodiesterase D]]
-[[Category: Arni, R K.]]
+[[Category: Arni, R K]]
-[[Category: Giuseppe, P O.]]
+[[Category: Giuseppe, P O]]
-[[Category: Murakami, M T.]]
+[[Category: Murakami, M T]]
-[[Category: Ullah, A.]]
+[[Category: Ullah, A]]
-[[Category: Veiga, S S.]]
+[[Category: Veiga, S S]]
 [[Category: Hydrolase]]
 [[Category: Inactive mutant h12a phospholipase d]]
 [[Category: Plc-like phosphodiesterase]]
 [[Category: Tim beta/alpha-barrel]]