1nb4: Difference between revisions
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'''HC-J4 RNA polymerase apo-form''' | {{Structure | ||
|PDB= 1nb4 |SIZE=350|CAPTION= <scene name='initialview01'>1nb4</scene>, resolution 2.0Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] | |||
|GENE= | |||
}} | |||
'''HC-J4 RNA polymerase apo-form''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1NB4 is a [ | 1NB4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_c_virus Hepatitis c virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NB4 OCA]. | ||
==Reference== | ==Reference== | ||
Substrate complexes of hepatitis C virus RNA polymerase (HC-J4): structural evidence for nucleotide import and de-novo initiation., O'Farrell D, Trowbridge R, Rowlands D, Jager J, J Mol Biol. 2003 Feb 28;326(4):1025-35. PMID:[http:// | Substrate complexes of hepatitis C virus RNA polymerase (HC-J4): structural evidence for nucleotide import and de-novo initiation., O'Farrell D, Trowbridge R, Rowlands D, Jager J, J Mol Biol. 2003 Feb 28;326(4):1025-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12589751 12589751] | ||
[[Category: Hepatitis c virus]] | [[Category: Hepatitis c virus]] | ||
[[Category: RNA-directed RNA polymerase]] | [[Category: RNA-directed RNA polymerase]] | ||
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[[Category: rna polymerase]] | [[Category: rna polymerase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:54:35 2008'' | ||
Revision as of 13:54, 20 March 2008
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| , resolution 2.0Å | |||||||
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| Activity: | RNA-directed RNA polymerase, with EC number 2.7.7.48 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HC-J4 RNA polymerase apo-form
OverviewOverview
Several crystal structures of the hepatitis C virus NS5B protein (genotype-1b, strain J4) complexed with metal ions, single-stranded RNA or nucleoside-triphosphates have been determined. These complexes illustrate how conserved amino acid side-chains, together with essential structural features within the active site, control nucleotide binding and likely mediate de-novo initiation. The incoming nucleotide interacts with several basic residues from an extension on the NS5B fingers domain, a beta-hairpin from the NS5B thumb domain and the C-terminal arm. The modular, bi-partite fingers domain carries a long binding groove which guides the template towards the catalytic site. The apo-polymerase structure provides unprecedented insights into potential non-nucleoside inhibitor binding sites located between palm and thumb near motif E, which is unique to RNA polymerases and reverse transcriptases.
About this StructureAbout this Structure
1NB4 is a Single protein structure of sequence from Hepatitis c virus. Full crystallographic information is available from OCA.
ReferenceReference
Substrate complexes of hepatitis C virus RNA polymerase (HC-J4): structural evidence for nucleotide import and de-novo initiation., O'Farrell D, Trowbridge R, Rowlands D, Jager J, J Mol Biol. 2003 Feb 28;326(4):1025-35. PMID:12589751
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