1n3p: Difference between revisions
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'''Pterocarpus angolensis lectin in complex with sucrose''' | {{Structure | ||
|PDB= 1n3p |SIZE=350|CAPTION= <scene name='initialview01'>1n3p</scene>, resolution 2.1Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Pterocarpus angolensis lectin in complex with sucrose''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1N3P is a [ | 1N3P is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Pterocarpus_angolensis Pterocarpus angolensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3P OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of Pterocarpus angolensis lectin in complex with glucose, sucrose, and turanose., Loris R, Imberty A, Beeckmans S, Van Driessche E, Read JS, Bouckaert J, De Greve H, Buts L, Wyns L, J Biol Chem. 2003 May 2;278(18):16297-303. Epub 2003 Feb 19. PMID:[http:// | Crystal structure of Pterocarpus angolensis lectin in complex with glucose, sucrose, and turanose., Loris R, Imberty A, Beeckmans S, Van Driessche E, Read JS, Bouckaert J, De Greve H, Buts L, Wyns L, J Biol Chem. 2003 May 2;278(18):16297-303. Epub 2003 Feb 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12595543 12595543] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Pterocarpus angolensis]] | [[Category: Pterocarpus angolensis]] | ||
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[[Category: sucrose]] | [[Category: sucrose]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:51:43 2008'' | ||
Revision as of 13:51, 20 March 2008
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| Ligands: | and | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Pterocarpus angolensis lectin in complex with sucrose
OverviewOverview
The crystal structure of the Man/Glc-specific seed lectin from Pterocarpus angolensis was determined in complex with methyl-alpha-d-glucose, sucrose, and turanose. The carbohydrate binding site contains a classic Man/Glc type specificity loop. Its metal binding loop on the other hand is of the long type, different from what is observed in other Man/Glc-specific legume lectins. Glucose binding in the primary binding site is reminiscent of the glucose complexes of concanavalin A and lentil lectin. Sucrose is found to be bound in a conformation similar as seen in the binding site of lentil lectin. A direct hydrogen bond between Ser-137(OG) to Fru(O2) in Pterocarpus angolensis lectin replaces a water-mediated interaction in the equivalent complex of lentil lectin. In the turanose complex, the binding site of the first molecule in the asymmetric unit contains the alphaGlc1-3betaFruf form of furanose while the second molecule contains the alphaGlc1-3betaFrup form in its binding site.
About this StructureAbout this Structure
1N3P is a Protein complex structure of sequences from Pterocarpus angolensis. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of Pterocarpus angolensis lectin in complex with glucose, sucrose, and turanose., Loris R, Imberty A, Beeckmans S, Van Driessche E, Read JS, Bouckaert J, De Greve H, Buts L, Wyns L, J Biol Chem. 2003 May 2;278(18):16297-303. Epub 2003 Feb 19. PMID:12595543
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