3mvd: Difference between revisions

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[[Image:3mvd.png|left|200px]]
==Crystal structure of the chromatin factor RCC1 in complex with the nucleosome core particle==
<StructureSection load='3mvd' size='340' side='right' caption='[[3mvd]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3mvd]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MVD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3MVD FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1kx5|1kx5]], [[1zla|1zla]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HISTONE H3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis]), HISTONE H4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis]), LOC494591 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis]), HISTONE H2B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8355 Xenopus laevis]), Bj1, CG10480, RCC1 (Bj1) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3mvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3mvd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3mvd RCSB], [http://www.ebi.ac.uk/pdbsum/3mvd PDBsum]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mv/3mvd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The small GTPase Ran enzyme regulates critical eukaryotic cellular functions including nuclear transport and mitosis through the creation of a RanGTP gradient around the chromosomes. This concentration gradient is created by the chromatin-bound RCC1 (regulator of chromosome condensation) protein, which recruits Ran to nucleosomes and activates Ran's nucleotide exchange activity. Although RCC1 has been shown to bind directly with the nucleosome, the molecular details of this interaction were not known. Here we determine the crystal structure of a complex of Drosophila RCC1 and the nucleosome core particle at 2.9 A resolution, providing an atomic view of how a chromatin protein interacts with the histone and DNA components of the nucleosome. Our structure also suggests that the Widom 601 DNA positioning sequence present in the nucleosomes forms a 145-base-pair nucleosome core particle, not the expected canonical 147-base-pair particle.


{{STRUCTURE_3mvd|  PDB=3mvd  |  SCENE=  }}
Structure of RCC1 chromatin factor bound to the nucleosome core particle.,Makde RD, England JR, Yennawar HP, Tan S Nature. 2010 Sep 30;467(7315):562-6. Epub 2010 Aug 25. PMID:20739938<ref>PMID:20739938</ref>


===Crystal structure of the chromatin factor RCC1 in complex with the nucleosome core particle===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_20739938}}
 
==About this Structure==
[[3mvd]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [http://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3MVD OCA].


==See Also==
==See Also==
*[[Histone|Histone]]
*[[Histone|Histone]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:020739938</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
[[Category: Xenopus laevis]]
[[Category: Xenopus laevis]]
[[Category: England, J R.]]
[[Category: England, J R]]
[[Category: Makde, R D.]]
[[Category: Makde, R D]]
[[Category: Tan, S.]]
[[Category: Tan, S]]
[[Category: Yennawar, H P.]]
[[Category: Yennawar, H P]]
[[Category: 7-bladed beta-propeller]]
[[Category: 7-bladed beta-propeller]]
[[Category: Ncp-chromatin factor complex]]
[[Category: Ncp-chromatin factor complex]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
[[Category: Signaling protein-structural protein-dna complex]]
[[Category: Signaling protein-structural protein-dna complex]]

Revision as of 13:00, 9 December 2014

Crystal structure of the chromatin factor RCC1 in complex with the nucleosome core particleCrystal structure of the chromatin factor RCC1 in complex with the nucleosome core particle

Structural highlights

3mvd is a 12 chain structure with sequence from Drosophila melanogaster and Xenopus laevis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:HISTONE H3 (Xenopus laevis), HISTONE H4 (Xenopus laevis), LOC494591 (Xenopus laevis), HISTONE H2B (Xenopus laevis), Bj1, CG10480, RCC1 (Bj1) (Drosophila melanogaster)
Resources:FirstGlance, OCA, RCSB, PDBsum

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The small GTPase Ran enzyme regulates critical eukaryotic cellular functions including nuclear transport and mitosis through the creation of a RanGTP gradient around the chromosomes. This concentration gradient is created by the chromatin-bound RCC1 (regulator of chromosome condensation) protein, which recruits Ran to nucleosomes and activates Ran's nucleotide exchange activity. Although RCC1 has been shown to bind directly with the nucleosome, the molecular details of this interaction were not known. Here we determine the crystal structure of a complex of Drosophila RCC1 and the nucleosome core particle at 2.9 A resolution, providing an atomic view of how a chromatin protein interacts with the histone and DNA components of the nucleosome. Our structure also suggests that the Widom 601 DNA positioning sequence present in the nucleosomes forms a 145-base-pair nucleosome core particle, not the expected canonical 147-base-pair particle.

Structure of RCC1 chromatin factor bound to the nucleosome core particle.,Makde RD, England JR, Yennawar HP, Tan S Nature. 2010 Sep 30;467(7315):562-6. Epub 2010 Aug 25. PMID:20739938[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Makde RD, England JR, Yennawar HP, Tan S. Structure of RCC1 chromatin factor bound to the nucleosome core particle. Nature. 2010 Sep 30;467(7315):562-6. Epub 2010 Aug 25. PMID:20739938 doi:10.1038/nature09321

3mvd, resolution 2.90Å

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