3lvg: Difference between revisions

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-[[Image:3lvg.png|left|200px]]
+==Crystal structure of a clathrin heavy chain and clathrin light chain complex==
+<StructureSection load='3lvg' size='340' side='right' caption='[[3lvg]], [[Resolution|resolution]] 7.94&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3lvg]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LVG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3LVG FirstGlance]. <br>
+</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3lvh|3lvh]]</td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CLTC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus]), CLTB, CLTLB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3lvg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3lvg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3lvg RCSB], [http://www.ebi.ac.uk/pdbsum/3lvg PDBsum]</span></td></tr>
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lv/3lvg_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Clathrin-coated vesicle formation is responsible for membrane traffic to and from the endocytic pathway during receptor-mediated endocytosis and organelle biogenesis, influencing how cells relate to their environment. Generating these vesicles involves self-assembly of clathrin molecules into a latticed coat on membranes that recruits receptors and organizes protein machinery necessary for budding. Here we define a molecular mechanism regulating clathrin lattice formation by obtaining structural information from co-crystals of clathrin subunits. Low resolution X-ray diffraction data (7.9-9.0 A) was analyzed using a combination of molecular replacement with an energy-minimized model and noncrystallographic symmetry averaging. Resulting topological information revealed two conformations of the regulatory clathrin light chain bound to clathrin heavy chain. Based on protein domain positions, mutagenesis, and biochemical assays, we identify an electrostatic interaction between the clathrin subunits that allows the observed conformational variation in clathrin light chains to alter the conformation of the clathrin heavy chain and thereby regulates assembly.
-{{STRUCTURE_3lvg|  PDB=3lvg  |  SCENE=  }}
+Conformation switching of clathrin light chain regulates clathrin lattice assembly.,Wilbur JD, Hwang PK, Ybe JA, Lane M, Sellers BD, Jacobson MP, Fletterick RJ, Brodsky FM Dev Cell. 2010 May 18;18(5):841-8. PMID:20493816<ref>PMID:20493816</ref>
-===Crystal structure of a clathrin heavy chain and clathrin light chain complex===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_20493816}}
-==About this Structure==
-[[3lvg]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3LVG OCA].
 ==See Also==
 *[[Clathrin|Clathrin]]
+== References ==
-==Reference==
+<references/>
-<ref group="xtra">PMID:020493816</ref><references group="xtra"/>
+__TOC__
+</StructureSection>
 [[Category: Bos taurus]]
-[[Category: Brodsky, F M.]]
+[[Category: Brodsky, F M]]
-[[Category: Fletterick, R J.]]
+[[Category: Fletterick, R J]]
-[[Category: Hwang, P K.]]
+[[Category: Hwang, P K]]
-[[Category: Jacobson, M P.]]
+[[Category: Jacobson, M P]]
-[[Category: Lane, M.]]
+[[Category: Lane, M]]
-[[Category: Sellers, B D.]]
+[[Category: Sellers, B D]]
-[[Category: Wilbur, J D.]]
+[[Category: Wilbur, J D]]
-[[Category: Ybe, J A.]]
+[[Category: Ybe, J A]]
 [[Category: Coated pit]]
 [[Category: Cytoplasmic vesicle]]