1mzt: Difference between revisions

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caption="1mzt" />
 
'''NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes'''<br />
{{Structure
|PDB= 1mzt |SIZE=350|CAPTION= <scene name='initialview01'>1mzt</scene>
|SITE=  
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'''NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes'''
 


==Overview==
==Overview==
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==About this Structure==
==About this Structure==
1MZT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Filamentous_phage Filamentous phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA].  
1MZT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Filamentous_phage Filamentous phage]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MZT OCA].  


==Reference==
==Reference==
Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints., Marassi FM, Opella SJ, Protein Sci. 2003 Mar;12(3):403-11. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12592011 12592011]
Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints., Marassi FM, Opella SJ, Protein Sci. 2003 Mar;12(3):403-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12592011 12592011]
[[Category: Filamentous phage]]
[[Category: Filamentous phage]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: pviii]]
[[Category: pviii]]


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:00:42 2008''
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Revision as of 13:50, 20 March 2008

File:1mzt.jpg


PDB ID 1mzt

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NMR structure of the fd bacteriophage pVIII coat protein in lipid bilayer membranes


OverviewOverview

A solid-state NMR approach for simultaneous resonance assignment and three-dimensional structure determination of a membrane protein in lipid bilayers is described. The approach is based on the scattering, hence the descriptor "shotgun," of (15)N-labeled amino acids throughout the protein sequence (and the resulting NMR spectra). The samples are obtained by protein expression in bacteria grown on media in which one type of amino acid is labeled and the others are not. Shotgun NMR short-circuits the laborious and time-consuming process of obtaining complete sequential assignments prior to the calculation of a protein structure from the NMR data by taking advantage of the orientational information inherent to the spectra of aligned proteins. As a result, it is possible to simultaneously assign resonances and measure orientational restraints for structure determination. A total of five two-dimensional (1)H/(15)N PISEMA (polarization inversion spin exchange at the magic angle) spectra, from one uniformly and four selectively (15)N-labeled samples, were sufficient to determine the structure of the membrane-bound form of the 50-residue major pVIII coat protein of fd filamentous bacteriophage. Pisa (polarity index slat angle) wheels are an essential element in the process, which starts with the simultaneous assignment of resonances and the assembly of isolated polypeptide segments, and culminates in the complete three-dimensional structure of the protein with atomic resolution. The principles are also applicable to weakly aligned proteins studied by solution NMR spectroscopy. [The structure we determined for the membrane-bound form of the Fd bacteriophage pVIII coat protein has been deposited in the Protein Data Bank as PDB file 1MZT.]

About this StructureAbout this Structure

1MZT is a Single protein structure of sequence from Filamentous phage. Full crystallographic information is available from OCA.

ReferenceReference

Simultaneous assignment and structure determination of a membrane protein from NMR orientational restraints., Marassi FM, Opella SJ, Protein Sci. 2003 Mar;12(3):403-11. PMID:12592011

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