1ml9: Difference between revisions
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[[Image:1ml9.gif|left|200px]] | [[Image:1ml9.gif|left|200px]] | ||
'''Structure of the Neurospora SET domain protein DIM-5, a histone lysine methyltransferase''' | {{Structure | ||
|PDB= 1ml9 |SIZE=350|CAPTION= <scene name='initialview01'>1ml9</scene>, resolution 1.98Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=UNK:UNKNOWN'>UNK</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Histone-lysine_N-methyltransferase Histone-lysine N-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.43 2.1.1.43] | |||
|GENE= | |||
}} | |||
'''Structure of the Neurospora SET domain protein DIM-5, a histone lysine methyltransferase''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1ML9 is a [ | 1ML9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ML9 OCA]. | ||
==Reference== | ==Reference== | ||
Structure of the Neurospora SET domain protein DIM-5, a histone H3 lysine methyltransferase., Zhang X, Tamaru H, Khan SI, Horton JR, Keefe LJ, Selker EU, Cheng X, Cell. 2002 Oct 4;111(1):117-27. PMID:[http:// | Structure of the Neurospora SET domain protein DIM-5, a histone H3 lysine methyltransferase., Zhang X, Tamaru H, Khan SI, Horton JR, Keefe LJ, Selker EU, Cheng X, Cell. 2002 Oct 4;111(1):117-27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12372305 12372305] | ||
[[Category: Histone-lysine N-methyltransferase]] | [[Category: Histone-lysine N-methyltransferase]] | ||
[[Category: Neurospora crassa]] | [[Category: Neurospora crassa]] | ||
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[[Category: dim-5]] | [[Category: dim-5]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:44:53 2008'' | ||
Revision as of 13:44, 20 March 2008
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| , resolution 1.98Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | and | ||||||
| Activity: | Histone-lysine N-methyltransferase, with EC number 2.1.1.43 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the Neurospora SET domain protein DIM-5, a histone lysine methyltransferase
OverviewOverview
AdoMet-dependent methylation of histones is part of the "histone code" that can profoundly influence gene expression. We describe the crystal structure of Neurospora DIM-5, a histone H3 lysine 9 methyltranferase (HKMT), determined at 1.98 A resolution, as well as results of biochemical characterization and site-directed mutagenesis of key residues. This SET domain protein bears no structural similarity to previously characterized AdoMet-dependent methyltransferases but includes notable features such as a triangular Zn3Cys9 zinc cluster in the pre-SET domain and a AdoMet binding site in the SET domain essential for methyl transfer. The structure suggests a mechanism for the methylation reaction and provides the structural basis for functional characterization of the HKMT family and the SET domain.
About this StructureAbout this Structure
1ML9 is a Single protein structure of sequence from Neurospora crassa. Full crystallographic information is available from OCA.
ReferenceReference
Structure of the Neurospora SET domain protein DIM-5, a histone H3 lysine methyltransferase., Zhang X, Tamaru H, Khan SI, Horton JR, Keefe LJ, Selker EU, Cheng X, Cell. 2002 Oct 4;111(1):117-27. PMID:12372305
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