Cas9: Difference between revisions
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[[Cas9]] is the RNA-guided [[DNA]] [[endonuclease]] used by the CRISPR(clustered regularly interspaced short palindromic repeats)-associated systems to generate double-strand DNA breaks in the invading DNA during an adaptive bacterial immune response. | [[Cas9]] is the RNA-guided [[DNA]] [[endonuclease]] used by the CRISPR(clustered regularly interspaced short palindromic repeats)-associated systems to generate double-strand DNA breaks in the invading DNA during an adaptive bacterial immune response. | ||
It has been exploited for use in genome editing systems. | It has been exploited for use in genome editing systems. In such systems, an engineered single-guide RNA (sgRNA) is used to target double-stranded breaks in genomic DNA. Depending on what repair pathway is triggered, often dictated by the inclusion of additional engineered components, the targeted site either is disrupted or incorporates additional genetic sequences. | ||
Articles in Proteopedia concerning Cas9 include: | Articles in Proteopedia concerning Cas9 include: | ||