Lipase: Difference between revisions

===Eukaryote lipase: ===

[[1hpl]] – hLip – horse <br />

[[1hlg]] – hLip – human - gastric<br />

[[3jw8]], [[3hju]] – mono-glyceride hLip<br />

[[1jmy]] – hBSSL  <br />

[[1akn]] – cBSSL – cattle <br />

[[2bce]] - cBSSL (mutant) <br />

[[1f6w]] - cBSSL – catalytic domain<br />

[[3o0d]] – Lip – ''Yarrowia lipolytica''<br />

[[4jei]] – YlLip (mutant)<br />

[[1gpl]] – Lip – Guinea pig<br />

[[3zpx]] – Lip – ''Ustilago maydis''<br />

===Prokaryote lipase: ===

[[3guu]], [[1lbs]], [[1lbt]], [[1tca]], [[1tcb]], [[1tcc]] – CaLipA – ''Candida antarctica''<br />

[[2veo]] – CaLipA – closed state<br />

[[4k6g]] – CaLipB<br />

[[3icv]], [[4k5q]], [[4k6h]], [[4k6k]] – CaLipB (mutant) <br />

[[1gz7]], [[1lpm]], [[1lps]]– CrLip 2 – ''Candida  rugosa'' - closed state<br />

[[1crl]], [[1trh]] – CrLip – open state<br />

[[1llf]] – Lip – ''Candida cylindracea''<br />

[[3g7n]] – Lip - ''Penicillium expansum''<br />

[[1tia]] - Lip – ''Penicillium camemberti''<br />

[[2qua]], [[2qub]] – LipA – ''Serratia marcescens''<br />

[[2hih]] – Lip – ''Staphylococcus hyicus''<br />

[[2fx5]] – Lip – ''Pseudomonas mendocina''<br />

[[1yzf]] – Lip – ''Enterococcus faecalis''<br />

[[1dt3]], [[1dt5]], [[1dte]], [[1du4]], [[1ein]], [[1tib]], [[4dyh]], [[4ea6]], [[4flf]], [[4gbg]], [[4gwl]] - TlLip - ''Thermomyces lanuginose''<br />

[[1jfr]] – Lip – ''Streptomyces exfoliates''<br />

[[1oil]] – BcLip - ''Burkholderia cepacia''<br />

[[2lip]] – BcLip – open state<br />

[[1cvl]] – Lip – ''Chromobacterium viscosum''<br />

[[1lgy]] – Lip II – ''Rhizopus niveus''<br />

[[1tic]] - Lip  – ''Rhizopus oryzae''<br />

[[1thg]] – Lip – ''Geotrichum candidum''<br />

[[3tgl]], [[4tgl]], [[1tgl]] – RmLip– ''Rhyzomucor miehei''<br />

[[2zvd]] – PsLip - ''Pseudomonas sp.'' – open state<br />

[[2z8x]] - PsLip – extracellular<br />

[[2zj6]], [[2zj7]] – PsLip (mutant) <br />

[[2z8z]] – PsLip(mutant) – closed state<br />

[[3lip]], [[3a6z]] - Lip - ''Pseudomonas cepacia'' – open state<br />

[[1qge]], [[1tah]] – Lip – ''Pseudomonas glumae''<br />

[[2w22]] – Lip – ''Geobacillus thermocatenulatus''<br />

[[1ji3]], [[1ku0]], [[4fmp]] – Lip – ''Bacillus stearothermophilus''<br />

[[1ah7]] - Lip – ''Bacillus cereus''<br />

[[2qxt]], [[2qxu]], [[1isp]], [[1i6w]], [[4fdm]] - BsLip  – ''Bacillus subtilis''<br />

[[3d2a]], [[3d2b]], [[3d2c]], [[1t2n]], [[1t4m]], [[3qmm]], [[3qzu]], [[4fkb]] - BsLip (mutant) <br />

[[2ory]] – Lip – ''Photobacterium lypoliticum''<br />

[[2z5g]], [[2dsn]] – GzLip T1 – ''Geobacillus zalihae''<br />

[[3umj]] – GzLip (mutant)<br />

[[3p94]] – Lip – ''Parabacteroides distasonis''<br />

[[3ngm]] – Lip – ''Gibberella zeae''<br />

[[3auk]] - Lip – ''Geobacillus''<br />

[[3uue]], [[3uuf]] – Lip – ''Malassezia globosa''<br />

[[4hs9]] – Lip – ''Proteus mirabilis''<br />

===Lipase/colipase complexes.  The colipase is a co-enzyme whose binding to lipase optimizes the enzymatic activity===

[[1n8s]] – hLip+colipase II<br />

[[1eth]], [[1lpa]] - Lip+colipase II - pig<br />

===Hormone-sensitive-lipases (LIPE) hydrolyze the first fatty acid of the triacylglycerol substrate===

[[3k6k]] – EstE7(LIPE) – metagenome library<br />

[[3fak]], [[3dnm]] – EstE5(LIPE) – metagenome library<br />

[[1evq]] – AaEst2(LIPE) – ''Alicyclobacillus acidocaldarius''<br />

[[1u4n]] – AaEst2(LIPE) (mutant) <br />

===Putative lipases; Proteins with unknown function but structural similarity to lipase obtained in structural genomics projects. ===

[[2rau]] - Lip – ''Sulfolobus solfataricus''<br />

[[3bxp]], [[3d3n]] - Lip – ''Lactobacillus plantarum''<br />

[[3e0x]] - Lip – ''Clostridium acetobutylicum''<br />

[[1z8h]] – Lip – ''Nostoc sp.'' PCC 712<br />

[[1vj3]] - Lip  – ''Nostoc sp.'' <br />

[[3bzw]] – Lip - ''Bacteroides thetaiotaomicron''<br />

[[2pbl]] – Lip - ''Silicibacter''

===Lipase + inhibitors===

[[3jwe]], [[3pe6]] - mono-glyceride hLip + SAR629 – covalent inhibitor<br />

[[3l1h]] – EstE5(LIPE)+FeCl3  – noninvasive inhibitor<br />

[[3l1i]], [[3l1j]] - EstE5(LIPE)+CuSO4 – noninvasive inhibitor<br />

[[3lij]] - EstE5(LIPE)+ZnSO4– noninvasive inhibitor<br />

[[3h18]], [[3h17]] - EstE5 (LIPE)+PMSF <br />

[[3h19]], [[3h1b]], [[3h1a]] – EstE5 (LIPE)+methyl alcohol<br />

[[3h1a]] – EstE5 SLIPE)+ethyl alcohol<br />

[[3h19]] – EstE5 SLIPE)+isopropyl alcohol<br />

[[3g9t]], [[3g9u]] - EstE5 (HSLIPE)+p-nitrophenyl butyrate<br />

[[3g9z]] - EstE5 (LIPE) +p-nitrophenyl caprylate<br />

[[2nw6]] – BcLip+ S inhibitor <br />

[[4lip]], [[5lip]], [[1r4z]], [[1r50]] – BcLip+ Rc-(Rp,Sp)-1,2-dioctylcarbamoyl-glycero-3-O-phosphonate<br />

[[1r4z]] – BsLip+Rc-IPG-phosphonate <br />

[[1r50]] - BsLip+Sc-IPG-phosphonate <br />

[[1k8q]] - Lip+phosphonate – dog <br />

[[1ex9]] – Lip+Rc-(Rp,Sp)-1,2-dioctylcarbamoyl-glycero-3-O-phosphonate – ''Pseudomonas aeruginosa'' <br />

[[5tgl]] – RmLip+N-hexyl-phosphonate <br />

[[1lpb]] – Lip (pig)+colipase+C11 alkyl phosphonate <br />

[[3icw]] – CaLipB (mutant) +methyl hydrogen R hexylphosphonate<br />

[[3a70]] – PsLip+diethyl phosphate<br />

[[4glb]] – TlLip + nitrobenzaldehyde<br />

[[4kjx]] - TlLip + nitrobenzaldehyde + lauric acid<br />

[[4n8s]] - TlLip + nitrobenzaldehyde + ethylacetoacetate<br />

===Lipase conjugated with analogs to its reaction intermediates===

[[1lpn]], [[1lpo]], [[1lpp]] – CrLip+ sulfonates <br />

[[3rar]] – CrLip+ phosphonate <br />

[[1qz3]] – EaEst2(mutant) (LIPE)+hexadecanesulfonate <br />

===Lipase showing bile-salt binding site===

[[1aql]] – cBSSL+taurocholate <br />

===Monoacylglycerol lipase ===

[[3rm3]], [[4lhe]] - BaMAGL – ''Bacillus'' <BR />

[[3hju]], [[3jw8]] - hMAGL  <BR />

[[3rli]] – BaMAGL + PMSF<br />

[[4ke7]], [[4ke8]], [[4ke9]] – BaMAGL + ligand<br />

[[4ke6]], [[4kea]] – BaMAGL (mutant) + ligand<br />

[[3jwe]], [[3pe6]] – hMAGL + inhibitor<br />

===Lipase with substrate bound at active site===

[[2zyh]] – AfLip (mutant)+fatty acid – ''Archaeoglobus fulgidus''<br />

[[2zyi]], [[2zyr]], [[2zys]] - AfLip+fatty acid+ ion <br />

[[1gt6]], [[4ghw]], [[4gi1]] – TlLip+ fatty acid - lipid ligand<br />

===Lipase conjugated to transition-state analogs showing the binding mode of the enzyme catalysis===

[[1ys1]] – BhLip+hexylphosphonic acid (R) 2-methyl-3-phenylpropyl ester <br />  

[[1ys2]] – BhLip+hexylphosphonic acid (S) 2-methyl-3-phenylpropyl ester<br />   

[[1hqd]] – Lip+1-phenoxy-2-acrtoxy butane – ''Pseudomonas cepacia'' <br />

===Lipase+lipase chaperone===