Insulin-Degrading Enzyme: Difference between revisions
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IDE-C is also involved in the oligomerization of IDE. | IDE-C is also involved in the oligomerization of IDE. | ||
== Catalytic chamber == | |||
IDE-N and IDE-C create substantial contact to form an enclosed catalytic chamber to encapsulate its substrates. This enclosed chamber is shaped like a triangular prism, with triangular base dimensions of 35x34x30 Ȧ and a height of 36 Ȧ. So the total volume of this prism is about 1.3x104 Ȧ<sup>3</sup>. | IDE-N and IDE-C create substantial contact to form an enclosed catalytic chamber to encapsulate its substrates. This enclosed chamber is shaped like a triangular prism, with triangular base dimensions of 35x34x30 Ȧ and a height of 36 Ȧ. So the total volume of this prism is about 1.3x104 Ȧ<sup>3</sup>. | ||
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In the domain 2, there is an exosite that accommodates the N-terminus of substrates and is involved in the substrates binding. | In the domain 2, there is an exosite that accommodates the N-terminus of substrates and is involved in the substrates binding. | ||
== Substrate binding == | |||
IDE can adopt two conformations, called “open” (IDE-o) and “closed” (IDE-c). | IDE can adopt two conformations, called “open” (IDE-o) and “closed” (IDE-c). | ||
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IDE-N and IDE-C have extensive interactions that bury a large surface of 11,496 Ȧ<sup>2</sup> with good shape complementarity and numerous hydrogen bonds. In the absence of interactions with factors or proteins, the substrate-free IDE-c state is stable and the catalytic chamber of IDE is mostly closed. So we can consider that IDE-c is the resting and inactive state of IDE. | IDE-N and IDE-C have extensive interactions that bury a large surface of 11,496 Ȧ<sup>2</sup> with good shape complementarity and numerous hydrogen bonds. In the absence of interactions with factors or proteins, the substrate-free IDE-c state is stable and the catalytic chamber of IDE is mostly closed. So we can consider that IDE-c is the resting and inactive state of IDE. | ||
== Han ethnicity-specific type 2 diabetic treatment from traditional Chinese medicine? <ref>doi 10.1080/07391102.2012.732340</ref>== | |||
Insulin-degrading enzyme (IDE) is a zinc-metalloendopeptidase found mostly in cytosol. Since overexpression of IDE increases the rate of extracellular insulin degradation, IDE is a potential target for controlling insulin degradation. | Insulin-degrading enzyme (IDE) is a zinc-metalloendopeptidase found mostly in cytosol. Since overexpression of IDE increases the rate of extracellular insulin degradation, IDE is a potential target for controlling insulin degradation. | ||
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</StructureSection> | </StructureSection> | ||
__NOTOC__ | __NOTOC__ | ||
==3D structures of insulin-degrading enzyme== | ==3D structures of insulin-degrading enzyme== | ||