3ewg: Difference between revisions

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-[[Image:3ewg.png|left|200px]]
+==Crystal structure of the N-terminal domain of NusG (NGN) from Methanocaldococcus jannaschii==
+<StructureSection load='3ewg' size='340' side='right' caption='[[3ewg]], [[Resolution|resolution]] 2.04&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3ewg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EWG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3EWG FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NusG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ewg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ewg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ewg RCSB], [http://www.ebi.ac.uk/pdbsum/3ewg PDBsum]</span></td></tr>
+</table>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ew/3ewg_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Transcription in archaea employs a eukaryotic-type transcription apparatus but uses bacterial-type transcription factors. NusG is one of the few archaeal transcription factors whose orthologs are essential in both bacteria and eukaryotes. Archaeal NusG is composed of only an NusG N-terminal (NGN) domain and a KOW domain, which is similar to bacterial NusG but not to the eukaryotic ortholog, Spt5. However, archaeal NusG was confirmed recently to form a complex with rpoE'' that was similar to the Spt5-Spt4 complex. Thus, archaeal NusG presents hybrid features of Spt5 and bacterial NusG. Here we report the crystal structure of NGN from the archaea Methanocaldococcus jannaschii (MjNGN). MjNGN folds to an alpha-beta-alpha sandwich without the appendant domain of bacterial NGNs, and forms a unique homodimer in crystal and solution. MjNGN alone was found to be sufficient for rpoE'' binding and an MjNGN-rpoE'' model has been constructed by rigid docking. Proteins 2009. (c) 2009 Wiley-Liss, Inc.
-{{STRUCTURE_3ewg|  PDB=3ewg  |  SCENE=  }}
+Crystal structure of NusG N-terminal (NGN) domain from Methanocaldococcus jannaschii and its interaction with rpoE'',Zhou H, Liu Q, Gao Y, Teng M, Niu L Proteins. 2009 Apr 28. PMID:19475703<ref>PMID:19475703</ref>
-===Crystal structure of the N-terminal domain of NusG (NGN) from Methanocaldococcus jannaschii===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_19475703}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[3ewg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EWG OCA].
+</StructureSection>
-==Reference==
-<ref group="xtra">PMID:019475703</ref><references group="xtra"/>
 [[Category: Methanocaldococcus jannaschii]]
-[[Category: Gao, Y X.]]
+[[Category: Gao, Y X]]
-[[Category: Liu, Q.]]
+[[Category: Liu, Q]]
-[[Category: Niu, L W.]]
+[[Category: Niu, L W]]
-[[Category: Teng, M K.]]
+[[Category: Teng, M K]]
-[[Category: Zhou, H H.]]
+[[Category: Zhou, H H]]
 [[Category: Alpha/beta structure]]
 [[Category: Antiparallel beta sheet]]
 [[Category: Flanked alpha helice]]
 [[Category: Transcription]]