1lz8: Difference between revisions

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Revision as of 13:36, 20 March 2008

File:1lz8.jpg

, resolution 1.53Å

Ligands:

and

Activity:

Lysozyme, with EC number 3.2.1.17

Coordinates:

save as pdb, mmCIF, xml

LYSOZYME PHASED ON ANOMALOUS SIGNAL OF SULFURS AND CHLORINES

OverviewOverview

A general method for solving the phase problem from native crystals of macromolecules has long eluded structural biology. For well diffracting crystals this goal can now be achieved, as is shown here, thanks to modern data collection techniques and new statistical phasing algorithms. Using solely a native crystal of tetragonal hen egg-white lysozyme, a protein of 14 kDa molecular mass, it was possible to detect the positions of the ten sulfur and seven chlorine atoms from their anomalous signal, and proceed from there to obtain an electron-density map of very high quality.

About this StructureAbout this Structure

1LZ8 is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.

ReferenceReference

Can anomalous signal of sulfur become a tool for solving protein crystal structures?, Dauter Z, Dauter M, de La Fortelle E, Bricogne G, Sheldrick GM, J Mol Biol. 1999 May 28;289(1):83-92. PMID:10339407

Page seeded by OCA on Thu Mar 20 12:36:55 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1lz8.jpg|left|200px]]<br /><applet load="1lz8" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1lz8.jpg|left|200px]]
-caption="1lz8, resolution 1.53&Aring;" />
-'''LYSOZYME PHASED ON ANOMALOUS SIGNAL OF SULFURS AND CHLORINES'''<br />
+ {{Structure
+|PDB= 1lz8 |SIZE=350|CAPTION= <scene name='initialview01'>1lz8</scene>, resolution 1.53&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene> and <scene name='pdbligand=NA:SODIUM ION'>NA</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]
+|GENE=
+}}
+'''LYSOZYME PHASED ON ANOMALOUS SIGNAL OF SULFURS AND CHLORINES'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-LZ8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] with <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LZ8 OCA].
+LZ8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LZ8 OCA].
 ==Reference==
-Can anomalous signal of sulfur become a tool for solving protein crystal structures?, Dauter Z, Dauter M, de La Fortelle E, Bricogne G, Sheldrick GM, J Mol Biol. 1999 May 28;289(1):83-92. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10339407 10339407]
+Can anomalous signal of sulfur become a tool for solving protein crystal structures?, Dauter Z, Dauter M, de La Fortelle E, Bricogne G, Sheldrick GM, J Mol Biol. 1999 May 28;289(1):83-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10339407 10339407]
 [[Category: Gallus gallus]]
 [[Category: Lysozyme]]
@@ Line 25: / Line 34: @@
 [[Category: o-glycosyl]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:49:52 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:36:55 2008''