1lto: Difference between revisions

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Revision as of 13:35, 20 March 2008

File:1lto.gif

, resolution 2.20Å

Activity:

Tryptase, with EC number 3.4.21.59

Coordinates:

save as pdb, mmCIF, xml

Human alpha1-tryptase

OverviewOverview

Human mast cell tryptases represent a subfamily of trypsin-like serine proteinases implicated in asthma. Unlike beta-tryptases, alpha-tryptases apparently are proteolytically inactive. We have solved the 2.2A crystal structure of mature human alpha1-tryptase. It reveals a frame-like tetrameric architecture that, surprisingly, does not require heparin-binding for stability. In marked contrast to beta2-tryptase, the Ser214-Gly219 segment, which normally provides the template for substrate binding, is kinked in alpha-tryptase, thereby blocking its non-primed subsites. This so far unobserved subsite distortion is incompatible with productive substrate binding and processing. alpha-Tryptase apparently is trapped in this off-conformation by repulsions and attractions of the Asp216 side-chain. However, proteolytic activity could be generated by an induced-fit mechanism.

About this StructureAbout this Structure

1LTO is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

The crystal structure of human alpha1-tryptase reveals a blocked substrate-binding region., Marquardt U, Zettl F, Huber R, Bode W, Sommerhoff C, J Mol Biol. 2002 Aug 16;321(3):491-502. PMID:12162961

Page seeded by OCA on Thu Mar 20 12:35:03 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1lto.gif|left|200px]]<br /><applet load="1lto" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1lto.gif|left|200px]]
-caption="1lto, resolution 2.20&Aring;" />
-'''Human alpha1-tryptase'''<br />
+ {{Structure
+|PDB= 1lto |SIZE=350|CAPTION= <scene name='initialview01'>1lto</scene>, resolution 2.20&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY= [http://en.wikipedia.org/wiki/Tryptase Tryptase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.59 3.4.21.59]
+|GENE=
+}}
+'''Human alpha1-tryptase'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-LTO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Tryptase Tryptase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.59 3.4.21.59] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTO OCA].
+LTO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LTO OCA].
 ==Reference==
-The crystal structure of human alpha1-tryptase reveals a blocked substrate-binding region., Marquardt U, Zettl F, Huber R, Bode W, Sommerhoff C, J Mol Biol. 2002 Aug 16;321(3):491-502. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12162961 12162961]
+The crystal structure of human alpha1-tryptase reveals a blocked substrate-binding region., Marquardt U, Zettl F, Huber R, Bode W, Sommerhoff C, J Mol Biol. 2002 Aug 16;321(3):491-502. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12162961 12162961]
 [[Category: Homo sapiens]]
 [[Category: Single protein]]
@@ Line 21: / Line 30: @@
 [[Category: hydrolase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:48:26 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:35:03 2008''