Endonuclease: Difference between revisions

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*HpyI ENN
*HpyI ENN


**[[3fc3]], [[3gox – HpENN99 + DNA – ''Helicobacter pylori''<br />
**[[3fc3]], [[3gox]] – HpENN99 + DNA – ''Helicobacter pylori''<br />
**[[3or3]] – HpENN88 + DNA
**[[3or3]] – HpENN88 + DNA


Revision as of 16:23, 24 November 2014

Template:STRUCTURE 1rva Endonuclease (ENN) cleaves phosphodiester bond within polynucleotide chain. ENN cleaves DNA at a restriction site which is usually a 6-nucleotide palindrome. ENN is restriction site–specific. Various types of ENN differ by their mechanism of action. ENN is used in genetic engineering to make recombinant DNA. ENN requires a restriction site and a cleavage pattern. ENN-I operates on DNA with separate restriction site and cleavage pattern, while ENN-II operates on overlapping restriction site and cleavage pattern. Some ENNs are encoded within introns thus facilitating their mobility. These ENNs or inteins are designated I-ENN. See also

3D structures of endonuclease3D structures of endonuclease

Updated on 24-November-2014

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Michal Harel, Alexander Berchansky, Joel L. Sussman
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