Pheromone binding protein: Difference between revisions
Nurit Eliash (talk | contribs) No edit summary |
Nurit Eliash (talk | contribs) No edit summary |
||
| Line 1: | Line 1: | ||
==Introduction== | ==Introduction== | ||
<StructureSection load='3bfa' size='340' side='right' caption='Pheromone binding protein of ''Apis mellifera'' scene=''> | <StructureSection load='3bfa' size='340' side='right' caption='Pheromone binding protein of ''Apis mellifera'' scene=''> | ||
Pheromone binding proteins [http://en.wikipedia.org/wiki/Pheromone_binding_protein (PBP)] are type of Odorant binding proteins [http://en.wikipedia.org/wiki/Odorant-binding_protein (OBP)] - soluble proteins mediating the early stages of volatiles detection in both insects and vertebrates | Pheromone binding proteins [http://en.wikipedia.org/wiki/Pheromone_binding_protein (PBP)] are type of Odorant binding proteins [http://en.wikipedia.org/wiki/Odorant-binding_protein (OBP)] - soluble proteins mediating the early stages of volatiles detection in both insects and vertebrates<ref>DOI:10.3389/fphys.2014.00320</ref>. | ||
<ref>DOI:10.3389/fphys.2014.00320</ref>. | |||
The volatiles (pheromones and other small hydrophobic molecules) are solubilized by the OBPs and activate the chemoreceptors. | The volatiles (pheromones and other small hydrophobic molecules) are solubilized by the OBPs and activate the chemoreceptors. | ||
As a model protein of this family we will further describe the well investigated Pheromone binding protein of the honey bee, ASP1. | As a model protein of this family we will further describe the well investigated Pheromone binding protein of the honey bee, ASP1. | ||
== Pheromone-binding protein ASP1 == | == Pheromone-binding protein ASP1 == | ||
Chemical communication is crucial in social insects, where a complicated and delicate system of signals must be maintained in order to preserve the fragile equilibrium between the queen and the workers. | Chemical communication is crucial in social insects, where a complicated and delicate system of signals must be maintained in order to preserve the fragile equilibrium between the queen and the workers. In the hive of the honey bee [http://en.wikipedia.org/wiki/Apis_mellifera ''Apis mellifera''] this equilibrium exists partially due to the extraction of blend of substances called queen mandibular pheromone [http://en.wikipedia.org/wiki/Honey_bee_pheromones#Queen_mandibular_pheromone (QMP)], by the queen <ref>Winston, M.L., 1987. The Biology of the Honey Bee. Harvard University Press, Cambridge, MA.</ref>. The three major component of the QMP blend are: 9-keto-2(E)-decenoic acid (9-ODA) and 9-hydroxy-2(E)-decenoic acid (9-HDA R-(−) or S-(+)). | ||
In the hive of the honey bee [http://en.wikipedia.org/wiki/Apis_mellifera ''Apis mellifera''] this equilibrium exists partially due to the extraction of blend of substances called queen mandibular pheromone [http://en.wikipedia.org/wiki/Honey_bee_pheromones#Queen_mandibular_pheromone (QMP)], by the queen <ref>Winston, M.L., 1987. The Biology of the Honey Bee. Harvard University Press, Cambridge, MA.</ref>. The three major component of the QMP blend are: 9-keto-2(E)-decenoic acid (9-ODA) and 9-hydroxy-2(E)-decenoic acid (9-HDA R-(−) or S-(+)). | |||
Pheromone-binding protein [http://www.uniprot.org/uniprot/Q9U9J6/ ASP] of the honeybee [http://en.wikipedia.org/wiki/Apis_mellifera ''Apis mellifera''] L. (Hymenoptera: Apidea) was first isolated and characterized by Danty ''et al''. (1998)<ref>DOI:10.1016/j.jmb.2008.04.048</ref> from the bee antennae. | Pheromone-binding protein [http://www.uniprot.org/uniprot/Q9U9J6/ ASP] of the honeybee [http://en.wikipedia.org/wiki/Apis_mellifera ''Apis mellifera''] L. (Hymenoptera: Apidea) was first isolated and characterized by Danty ''et al''. (1998)<ref>DOI:10.1016/j.jmb.2008.04.048</ref> from the bee antennae. | ||
== Structure == | == Structure == | ||
| Line 20: | Line 15: | ||
== Interaction with the ligand 9-ODA== | == Interaction with the ligand 9-ODA== | ||
<scene name='60/609542/9-oda/3'>9-ODA</scene> | <scene name='60/609542/9-oda/3'>9-ODA</scene> | ||
The carboxyl end of | The component 9-ODA is binding to the binding site with a <scene name='60/609542/Glycerol/2'>glycerol molecule</scene>. | ||
The carboxyl end of 9-ODA points towards the solvent, and has no bonds with residues of the protein. The residues in the binding site are <scene name='60/609542/Binding_site/3'>hydrophobic</scene> | |||
, and the connection between 9-ODA and ASP1 involve hydrogen bonds. | , and the connection between 9-ODA and ASP1 involve hydrogen bonds. | ||
== References == | == References == | ||
<references/> | <references/> | ||