1llm: Difference between revisions

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Revision as of 13:32, 20 March 2008

File:1llm.jpg

, resolution 1.50Å

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Crystal Structure of a Zif23-GCN4 Chimera Bound to DNA

OverviewOverview

Proteins that employ dimerization domains to bind cooperatively to DNA have a number of potential advantages over monomers with regards to gene regulation. Using a combination of structure-based design and phage display, a dimeric Cys(2)His(2) zinc finger protein has been created that binds cooperatively to DNA via an attached leucine zipper dimerization domain. This chimera, derived from components of Zif268 and GCN4, displayed excellent DNA-binding specificity, and we now report the 1.5 A resolution cocrystal structure of the Zif268-GCN4 homodimer bound to DNA. This structure shows how phage display has annealed the DNA binding and dimerization domains into a single functional unit. Moreover, this chimera provides a potential platform for the creation heterodimeric zinc finger proteins that can regulate a desired target gene through cooperative DNA recognition.

About this StructureAbout this Structure

1LLM is a Single protein structure of sequence from Mus musculus and saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

Structure of a designed dimeric zinc finger protein bound to DNA., Wolfe SA, Grant RA, Pabo CO, Biochemistry. 2003 Nov 25;42(46):13401-9. PMID:14621985

Page seeded by OCA on Thu Mar 20 12:32:18 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1llm.jpg|left|200px]]<br /><applet load="1llm" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1llm.jpg|left|200px]]
-caption="1llm, resolution 1.50&Aring;" />
-'''Crystal Structure of a Zif23-GCN4 Chimera Bound to DNA'''<br />
+ {{Structure
+|PDB= 1llm |SIZE=350|CAPTION= <scene name='initialview01'>1llm</scene>, resolution 1.50&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''Crystal Structure of a Zif23-GCN4 Chimera Bound to DNA'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-LLM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus_and_saccharomyces_cerevisiae Mus musculus and saccharomyces cerevisiae] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LLM OCA].
+LLM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus_and_saccharomyces_cerevisiae Mus musculus and saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LLM OCA].
 ==Reference==
-Structure of a designed dimeric zinc finger protein bound to DNA., Wolfe SA, Grant RA, Pabo CO, Biochemistry. 2003 Nov 25;42(46):13401-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14621985 14621985]
+Structure of a designed dimeric zinc finger protein bound to DNA., Wolfe SA, Grant RA, Pabo CO, Biochemistry. 2003 Nov 25;42(46):13401-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14621985 14621985]
 [[Category: Mus musculus and saccharomyces cerevisiae]]
 [[Category: Single protein]]
@@ Line 22: / Line 31: @@
 [[Category: structure-based design]]
 [[Category: x-ray crystallography]]
-[[Category: zinc fingers]]
+[[Category: zinc finger]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:46:09 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:32:18 2008''