1ll5: Difference between revisions

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Revision as of 13:32, 20 March 2008

File:1ll5.gif

, resolution 1.80Å

Ligands:

Gene:

K12 (Escherichia coli)

Activity:

Beta-lactamase, with EC number 3.5.2.6

Coordinates:

save as pdb, mmCIF, xml

X-ray crystal structure of AmpC WT beta-lactamase in complex with covalently bound imipenem

OverviewOverview

To determine how imipenem inhibits the class C beta-lactamase AmpC, the X-ray crystal structure of the acyl-enzyme complex was determined to a resolution of 1.80 A. In the complex, the lactam carbonyl oxygen of imipenem has flipped by approximately 180 degrees compared to its expected position; the electrophilic acyl center is thus displaced from the point of hydrolytic attack. This conformation resembles that of imipenem bound to the class A enzyme TEM-1 but is different from that of moxalactam bound to AmpC.

About this StructureAbout this Structure

1LL5 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Structural basis for imipenem inhibition of class C beta-lactamases., Beadle BM, Shoichet BK, Antimicrob Agents Chemother. 2002 Dec;46(12):3978-80. PMID:12435704

Page seeded by OCA on Thu Mar 20 12:32:07 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1ll5.gif|left|200px]]<br /><applet load="1ll5" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1ll5.gif|left|200px]]
-caption="1ll5, resolution 1.80&Aring;" />
-'''X-ray crystal structure of AmpC WT beta-lactamase in complex with covalently bound imipenem'''<br />
+ {{Structure
+|PDB= 1ll5 |SIZE=350|CAPTION= <scene name='initialview01'>1ll5</scene>, resolution 1.80&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=IM2:3-(2-FORMIMIDOYLAMINO-ETHYLSULFANYL)-5-(1-FORMYL-2-HYDROXY-PROPYL)-4,5-DIHYDRO-1H-PYRROLE-2-CARBOXYLIC ACID'>IM2</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]
+|GENE= K12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
+}}
+'''X-ray crystal structure of AmpC WT beta-lactamase in complex with covalently bound imipenem'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-LL5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=IM2:'>IM2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LL5 OCA].
+LL5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LL5 OCA].
 ==Reference==
-Structural basis for imipenem inhibition of class C beta-lactamases., Beadle BM, Shoichet BK, Antimicrob Agents Chemother. 2002 Dec;46(12):3978-80. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12435704 12435704]
+Structural basis for imipenem inhibition of class C beta-lactamases., Beadle BM, Shoichet BK, Antimicrob Agents Chemother. 2002 Dec;46(12):3978-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12435704 12435704]
 [[Category: Beta-lactamase]]
 [[Category: Escherichia coli]]
@@ Line 21: / Line 30: @@
 [[Category: imipenem]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:45:57 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:32:07 2008''