1lb1: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1lb1.jpg|left|200px]] | [[Image:1lb1.jpg|left|200px]] | ||
'''Crystal Structure of the Dbl and Pleckstrin homology domains of Dbs in complex with RhoA''' | {{Structure | ||
|PDB= 1lb1 |SIZE=350|CAPTION= <scene name='initialview01'>1lb1</scene>, resolution 2.81Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= Dbs ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]), RhoA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''Crystal Structure of the Dbl and Pleckstrin homology domains of Dbs in complex with RhoA''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1LB1 is a [ | 1LB1 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LB1 OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis for the selective activation of Rho GTPases by Dbl exchange factors., Snyder JT, Worthylake DK, Rossman KL, Betts L, Pruitt WM, Siderovski DP, Der CJ, Sondek J, Nat Struct Biol. 2002 Jun;9(6):468-75. PMID:[http:// | Structural basis for the selective activation of Rho GTPases by Dbl exchange factors., Snyder JT, Worthylake DK, Rossman KL, Betts L, Pruitt WM, Siderovski DP, Der CJ, Sondek J, Nat Struct Biol. 2002 Jun;9(6):468-75. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12006984 12006984] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
| Line 22: | Line 31: | ||
[[Category: Sondek, J.]] | [[Category: Sondek, J.]] | ||
[[Category: Worthylake, D K.]] | [[Category: Worthylake, D K.]] | ||
[[Category: | [[Category: db]] | ||
[[Category: dh domain]] | [[Category: dh domain]] | ||
[[Category: guanine nucleotide exchange factor]] | [[Category: guanine nucleotide exchange factor]] | ||
| Line 29: | Line 38: | ||
[[Category: small g-protein]] | [[Category: small g-protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:28:44 2008'' | ||
Revision as of 13:28, 20 March 2008
| |||||||
| , resolution 2.81Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | Dbs (Mus musculus), RhoA (Homo sapiens) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the Dbl and Pleckstrin homology domains of Dbs in complex with RhoA
OverviewOverview
Activation of Rho-family GTPases involves the removal of bound GDP and the subsequent loading of GTP, all catalyzed by guanine nucleotide exchange factors (GEFs) of the Dbl-family. Despite high sequence conservation among Rho GTPases, Dbl proteins possess a wide spectrum of discriminatory potentials for Rho-family members. To rationalize this specificity, we have determined crystal structures of the conserved, catalytic fragments (Dbl and pleckstrin homology domains) of the exchange factors intersectin and Dbs in complex with their cognate GTPases, Cdc42 and RhoA, respectively. Structure-based mutagenesis of intersectin and Dbs reveals the key determinants responsible for promoting exchange activity in Cdc42, Rac1 and RhoA. These findings provide critical insight into the structural features necessary for the proper pairing of Dbl-exchange factors with Rho GTPases and now allow for the detailed manipulation of signaling pathways mediated by these oncoproteins in vivo.
About this StructureAbout this Structure
1LB1 is a Protein complex structure of sequences from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for the selective activation of Rho GTPases by Dbl exchange factors., Snyder JT, Worthylake DK, Rossman KL, Betts L, Pruitt WM, Siderovski DP, Der CJ, Sondek J, Nat Struct Biol. 2002 Jun;9(6):468-75. PMID:12006984
Page seeded by OCA on Thu Mar 20 12:28:44 2008