1l2h: Difference between revisions
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'''Crystal structure of Interleukin 1-beta F42W/W120F mutant''' | {{Structure | ||
|PDB= 1l2h |SIZE=350|CAPTION= <scene name='initialview01'>1l2h</scene>, resolution 1.54Å | |||
|SITE= | |||
|LIGAND= | |||
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'''Crystal structure of Interleukin 1-beta F42W/W120F mutant''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1L2H is a [ | 1L2H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L2H OCA]. | ||
==Reference== | ==Reference== | ||
Use of multiple anomalous dispersion to phase highly merohedrally twinned crystals of interleukin-1beta., Rudolph MG, Kelker MS, Schneider TR, Yeates TO, Oseroff V, Heidary DK, Jennings PA, Wilson IA, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):290-8. Epub 2003, Jan 23. PMID:[http:// | Use of multiple anomalous dispersion to phase highly merohedrally twinned crystals of interleukin-1beta., Rudolph MG, Kelker MS, Schneider TR, Yeates TO, Oseroff V, Heidary DK, Jennings PA, Wilson IA, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):290-8. Epub 2003, Jan 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12554939 12554939] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein folding]] | [[Category: protein folding]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:25:26 2008'' | ||
Revision as of 13:25, 20 March 2008
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Crystal structure of Interleukin 1-beta F42W/W120F mutant
OverviewOverview
The crystal structure at 1.54 A resolution of a double mutant of interleukin-1beta (F42W/W120F), a cytokine secreted by macrophages, was determined by multiple-wavelength anomalous dispersion (MAD) using data from highly twinned selenomethionine-modified crystals. The space group is P4(3), with unit-cell parameters a = b = 53.9, c = 77.4 A. Self-rotation function analysis and various intensity statistics revealed the presence of merohedral twinning in crystals of both the native (twinning fraction alpha approximately 0.35) and SeMet (alpha approximately 0.40) forms. Structure determination and refinement are discussed with emphasis on the possible reasons for successful phasing using untreated twinned MAD data.
DiseaseDisease
Known disease associated with this structure: Gastric cancer risk after H. pylori infection OMIM:[147720]
About this StructureAbout this Structure
1L2H is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Use of multiple anomalous dispersion to phase highly merohedrally twinned crystals of interleukin-1beta., Rudolph MG, Kelker MS, Schneider TR, Yeates TO, Oseroff V, Heidary DK, Jennings PA, Wilson IA, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):290-8. Epub 2003, Jan 23. PMID:12554939
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