1l0h: Difference between revisions

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Revision as of 13:24, 20 March 2008

File:1l0h.jpg

, resolution 2.0Å

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CRYSTAL STRUCTURE OF BUTYRYL-ACP FROM E.COLI

OverviewOverview

Acyl carrier protein (ACP) is an essential cofactor in biosynthesis of fatty acids and many other reactions that require acyl transfer steps. We have determined the first crystal structures of an acylated form of ACP from E. coli, that of butyryl-ACP. Our analysis of the molecular surface of ACP reveals a plastic hydrophobic cavity in the vicinity of the phosphopantethylated Ser36 residue that is expanded and occupied by the butyryl and beta-mercaptoethylamine moieties of the acylated 4'-phosphopantetheine group in one of our crystal forms. In the other form, the cavity is contracted, and we propose that the protein has adopted the conformation after delivery of substrate into the active site of a partner enzyme.

About this StructureAbout this Structure

1L0H is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

X-ray crystallographic studies on butyryl-ACP reveal flexibility of the structure around a putative acyl chain binding site., Roujeinikova A, Baldock C, Simon WJ, Gilroy J, Baker PJ, Stuitje AR, Rice DW, Slabas AR, Rafferty JB, Structure. 2002 Jun;10(6):825-35. PMID:12057197

Page seeded by OCA on Thu Mar 20 12:24:39 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1l0h.jpg|left|200px]]<br /><applet load="1l0h" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1l0h.jpg|left|200px]]
-caption="1l0h, resolution 2.0&Aring;" />
-'''CRYSTAL STRUCTURE OF BUTYRYL-ACP FROM E.COLI'''<br />
+ {{Structure
+|PDB= 1l0h |SIZE=350|CAPTION= <scene name='initialview01'>1l0h</scene>, resolution 2.0&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''CRYSTAL STRUCTURE OF BUTYRYL-ACP FROM E.COLI'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-L0H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L0H OCA].
+L0H is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L0H OCA].
 ==Reference==
-X-ray crystallographic studies on butyryl-ACP reveal flexibility of the structure around a putative acyl chain binding site., Roujeinikova A, Baldock C, Simon WJ, Gilroy J, Baker PJ, Stuitje AR, Rice DW, Slabas AR, Rafferty JB, Structure. 2002 Jun;10(6):825-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12057197 12057197]
+X-ray crystallographic studies on butyryl-ACP reveal flexibility of the structure around a putative acyl chain binding site., Roujeinikova A, Baldock C, Simon WJ, Gilroy J, Baker PJ, Stuitje AR, Rice DW, Slabas AR, Rafferty JB, Structure. 2002 Jun;10(6):825-35. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12057197 12057197]
 [[Category: Escherichia coli]]
 [[Category: Single protein]]
@@ Line 28: / Line 37: @@
 [[Category: fatty acid biosynthesis]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:39:59 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:24:39 2008''