1kqq: Difference between revisions
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[[Image:1kqq.gif|left|200px]] | [[Image:1kqq.gif|left|200px]] | ||
'''Solution Structure of the Dead ringer ARID-DNA Complex''' | {{Structure | ||
|PDB= 1kqq |SIZE=350|CAPTION= <scene name='initialview01'>1kqq</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= dri ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]) | |||
}} | |||
'''Solution Structure of the Dead ringer ARID-DNA Complex''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1KQQ is a [ | 1KQQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KQQ OCA]. | ||
==Reference== | ==Reference== | ||
The structure of the Dead ringer-DNA complex reveals how AT-rich interaction domains (ARIDs) recognize DNA., Iwahara J, Iwahara M, Daughdrill GW, Ford J, Clubb RT, EMBO J. 2002 Mar 1;21(5):1197-209. PMID:[http:// | The structure of the Dead ringer-DNA complex reveals how AT-rich interaction domains (ARIDs) recognize DNA., Iwahara J, Iwahara M, Daughdrill GW, Ford J, Clubb RT, EMBO J. 2002 Mar 1;21(5):1197-209. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11867548 11867548] | ||
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:20:56 2008'' | ||
Revision as of 13:20, 20 March 2008
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| Gene: | dri (Drosophila melanogaster) | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of the Dead ringer ARID-DNA Complex
OverviewOverview
The AT-rich interaction domain (ARID) is a DNA-binding module found in many eukaryotic transcription factors. Using NMR spectroscopy, we have determined the first ever three-dimensional structure of an ARID--DNA complex (mol. wt 25.7 kDa) formed by Dead ringer from Drosophila melanogaster. ARIDs recognize DNA through a novel mechanism involving major groove immobilization of a large loop that connects the helices of a non-canonical helix-turn-helix motif, and through a concomitant structural rearrangement that produces stabilizing contacts from a beta-hairpin. Dead ringer's preference for AT-rich DNA originates from three positions within the ARID fold that form energetically significant contacts to an adenine-thymine base step. Amino acids that dictate binding specificity are not highly conserved, suggesting that ARIDs will bind to a range of nucleotide sequences. Extended ARIDs, found in several sequence-specific transcription factors, are distinguished by the presence of a C-terminal helix that may increase their intrinsic affinity for DNA. The prevalence of serine amino acids at all specificity determining positions suggests that ARIDs within SWI/SNF-related complexes will interact with DNA non-sequence specifically.
About this StructureAbout this Structure
1KQQ is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
ReferenceReference
The structure of the Dead ringer-DNA complex reveals how AT-rich interaction domains (ARIDs) recognize DNA., Iwahara J, Iwahara M, Daughdrill GW, Ford J, Clubb RT, EMBO J. 2002 Mar 1;21(5):1197-209. PMID:11867548
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