1kio: Difference between revisions
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'''SOLUTION STRUCTURE OF THE SMALL SERINE PROTEASE INHIBITOR SGCI[L30R, K31M]''' | {{Structure | ||
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'''SOLUTION STRUCTURE OF THE SMALL SERINE PROTEASE INHIBITOR SGCI[L30R, K31M]''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1KIO is a [ | 1KIO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIO OCA]. | ||
==Reference== | ==Reference== | ||
Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria., Gaspari Z, Patthy A, Graf L, Perczel A, Eur J Biochem. 2002 Jan;269(2):527-37. PMID:[http:// | Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria., Gaspari Z, Patthy A, Graf L, Perczel A, Eur J Biochem. 2002 Jan;269(2):527-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11856311 11856311] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Gaspari, Z.]] | [[Category: Gaspari, Z.]] | ||
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[[Category: specificity]] | [[Category: specificity]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:17:57 2008'' | ||
Revision as of 13:17, 20 March 2008
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SOLUTION STRUCTURE OF THE SMALL SERINE PROTEASE INHIBITOR SGCI[L30R, K31M]
OverviewOverview
The solution structure of three small serine proteinase inhibitors, two natural and one engineered protein, SGCI (Schistocerca gregaria chymotrypsin inhibitor), SGCI[L30R, K31M] and SGTI (Schistocerca gregaria trypsin inhibitor), were determined by homonuclear NMR-spectroscopy. The molecules exhibit different specificities towards target proteinases, where SGCI is a good chymotrypsin inhibitor, its mutant is a potent trypsin inhibitor, and SGTI inhibits both proteinases weakly. Interestingly, SGTI is a much better inhibitor of insect proteinases than of the mammalian ones used in common assays. All three molecules have a similar fold composed from three antiparallel beta-pleated sheets with three disulfide bridges. The proteinase binding loop has a somewhat distinct geometry in all three peptides. Moreover, the stabilization of the structure is different in SGCI and SGTI. Proton-deuterium exchange experiments are indicative of a highly rigid core in SGTI but not in SGCI. We suggest that the observed structural properties play a significant role in the specificity of these inhibitors.
About this StructureAbout this Structure
1KIO is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria., Gaspari Z, Patthy A, Graf L, Perczel A, Eur J Biochem. 2002 Jan;269(2):527-37. PMID:11856311
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