1khc: Difference between revisions
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'''Crystal Structure of the PWWP Domain of Mammalian DNA Methyltransferase Dnmt3b''' | {{Structure | ||
|PDB= 1khc |SIZE=350|CAPTION= <scene name='initialview01'>1khc</scene>, resolution 1.8Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=UNX:UNKNOWN ATOM OR ION'>UNX</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA_(cytosine-5-)-methyltransferase DNA (cytosine-5-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.37 2.1.1.37] | |||
|GENE= Dnmt3b ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus]) | |||
}} | |||
'''Crystal Structure of the PWWP Domain of Mammalian DNA Methyltransferase Dnmt3b''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1KHC is a [ | 1KHC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHC OCA]. | ||
==Reference== | ==Reference== | ||
The PWWP domain of mammalian DNA methyltransferase Dnmt3b defines a new family of DNA-binding folds., Qiu C, Sawada K, Zhang X, Cheng X, Nat Struct Biol. 2002 Mar;9(3):217-24. PMID:[http:// | The PWWP domain of mammalian DNA methyltransferase Dnmt3b defines a new family of DNA-binding folds., Qiu C, Sawada K, Zhang X, Cheng X, Nat Struct Biol. 2002 Mar;9(3):217-24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11836534 11836534] | ||
[[Category: DNA (cytosine-5-)-methyltransferase]] | [[Category: DNA (cytosine-5-)-methyltransferase]] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
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[[Category: five beta-sheets barrel followed by five-helix bundle]] | [[Category: five beta-sheets barrel followed by five-helix bundle]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:17:29 2008'' | ||
Revision as of 13:17, 20 March 2008
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| , resolution 1.8Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | Dnmt3b (Mus musculus) | ||||||
| Activity: | DNA (cytosine-5-)-methyltransferase, with EC number 2.1.1.37 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the PWWP Domain of Mammalian DNA Methyltransferase Dnmt3b
OverviewOverview
The PWWP domain is a weakly conserved sequence motif found in > 60 eukaryotic proteins, including the mammalian DNA methyltransferases Dnmt3a and Dnmt3b. These proteins often contain other chromatin-association domains. A 135-residue PWWP domain from mouse Dnmt3b (amino acids 223--357) has been structurally characterized at 1.8 A resolution. The N-terminal half of this domain resembles a barrel-like five-stranded structure, whereas the C-terminal half contains a five-helix bundle. The two halves are packed against each other to form a single structural module that exhibits a prominent positive electrostatic potential. The PWWP domain alone binds DNA in vitro, probably through its basic surface. We also show that recombinant Dnmt3b2 protein (a splice variant of Dnmt3b) and two N-terminal deletion mutants (Delta218 and Delta369) have approximately equal methyl transfer activity on unmethylated and hemimethylated CpG-containing oligonucleotides. The Delta218 protein, which includes the PWWP domain, binds DNA more strongly than Delta369, which lacks the PWWP domain.
About this StructureAbout this Structure
1KHC is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
ReferenceReference
The PWWP domain of mammalian DNA methyltransferase Dnmt3b defines a new family of DNA-binding folds., Qiu C, Sawada K, Zhang X, Cheng X, Nat Struct Biol. 2002 Mar;9(3):217-24. PMID:11836534
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