1khb: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1khb.jpg|left|200px]] | [[Image:1khb.jpg|left|200px]] | ||
'''PEPCK complex with nonhydrolyzable GTP analog, native data''' | {{Structure | ||
|PDB= 1khb |SIZE=350|CAPTION= <scene name='initialview01'>1khb</scene>, resolution 1.854Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene> and <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxykinase_(GTP) Phosphoenolpyruvate carboxykinase (GTP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.32 4.1.1.32] | |||
|GENE= PCK1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''PEPCK complex with nonhydrolyzable GTP analog, native data''' | |||
==Overview== | ==Overview== | ||
| Line 10: | Line 19: | ||
==About this Structure== | ==About this Structure== | ||
1KHB is a [ | 1KHB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KHB OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site., Dunten P, Belunis C, Crowther R, Hollfelder K, Kammlott U, Levin W, Michel H, Ramsey GB, Swain A, Weber D, Wertheimer SJ, J Mol Biol. 2002 Feb 15;316(2):257-64. PMID:[http:// | Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site., Dunten P, Belunis C, Crowther R, Hollfelder K, Kammlott U, Levin W, Michel H, Ramsey GB, Swain A, Weber D, Wertheimer SJ, J Mol Biol. 2002 Feb 15;316(2):257-64. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11851336 11851336] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Phosphoenolpyruvate carboxykinase (GTP)]] | [[Category: Phosphoenolpyruvate carboxykinase (GTP)]] | ||
| Line 35: | Line 44: | ||
[[Category: p-loop]] | [[Category: p-loop]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:17:30 2008'' | ||
Revision as of 13:17, 20 March 2008
| |||||||
| , resolution 1.854Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Gene: | PCK1 (Homo sapiens) | ||||||
| Activity: | Phosphoenolpyruvate carboxykinase (GTP), with EC number 4.1.1.32 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
PEPCK complex with nonhydrolyzable GTP analog, native data
OverviewOverview
We report crystal structures of the human enzyme phosphoenolpyruvate carboxykinase (PEPCK) with and without bound substrates. These structures are the first to be determined for a GTP-dependent PEPCK, and provide the first view of a novel GTP-binding site unique to the GTP-dependent PEPCK family. Three phenylalanine residues form the walls of the guanine-binding pocket on the enzyme's surface and, most surprisingly, one of the phenylalanine side-chains contributes to the enzyme's specificity for GTP. PEPCK catalyzes the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Because the gluconeogenic pathway contributes to the fasting hyperglycemia of type II diabetes, inhibitors of PEPCK may be useful in the treatment of diabetes.
DiseaseDisease
Known disease associated with this structure: Hypoglycemia due to PCK1 deficiency (1) OMIM:[261680]
About this StructureAbout this Structure
1KHB is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site., Dunten P, Belunis C, Crowther R, Hollfelder K, Kammlott U, Levin W, Michel H, Ramsey GB, Swain A, Weber D, Wertheimer SJ, J Mol Biol. 2002 Feb 15;316(2):257-64. PMID:11851336
Page seeded by OCA on Thu Mar 20 12:17:30 2008