1kfr: Difference between revisions

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Revision as of 13:16, 20 March 2008

File:1kfr.jpg

, resolution 1.85Å

Ligands:

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Structural plasticity in the eight-helix fold of a trematode hemoglobin

OverviewOverview

The three-dimensional structure of recombinant haemoglobin from the trematode Paramphistomum epiclitum, displaying the highest oxygen affinity so far observed for (non)vertebrate haemoglobins, has previously been determined at 1.17 A resolution (orthorhombic space group P2(1)2(1)2(1)). In the present communication, the three-dimensional structure of wild-type P. epiclitum haemoglobin is reported at 1.85 A resolution in a monoclinic crystal form (R factor = 16.1%, R(free) = 22.0%). Comparison of P. epiclitum (recombinant versus wild-type ferric Hb) structures in the two crystal forms shows structural differences in the haem proximal and distal sites which have not been reported for other known haemoglobin structures previously.

About this StructureAbout this Structure

1KFR is a Single protein structure of sequence from Paramphistomum epiclitum. Full crystallographic information is available from OCA.

ReferenceReference

Structural plasticity in the eight-helix fold of a trematode haemoglobin., Milani M, Pesce A, Dewilde S, Ascenzi P, Moens L, Bolognesi M, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):719-22. Epub 2002, Mar 22. PMID:11914507

Page seeded by OCA on Thu Mar 20 12:16:53 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1kfr.jpg|left|200px]]<br /><applet load="1kfr" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1kfr.jpg|left|200px]]
-caption="1kfr, resolution 1.85&Aring;" />
-'''Structural plasticity in the eight-helix fold of a trematode hemoglobin'''<br />
+ {{Structure
+|PDB= 1kfr |SIZE=350|CAPTION= <scene name='initialview01'>1kfr</scene>, resolution 1.85&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=HEM:PROTOPORPHYRIN IX CONTAINING FE'>HEM</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''Structural plasticity in the eight-helix fold of a trematode hemoglobin'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-KFR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Paramphistomum_epiclitum Paramphistomum epiclitum] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KFR OCA].
+KFR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Paramphistomum_epiclitum Paramphistomum epiclitum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KFR OCA].
 ==Reference==
-Structural plasticity in the eight-helix fold of a trematode haemoglobin., Milani M, Pesce A, Dewilde S, Ascenzi P, Moens L, Bolognesi M, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):719-22. Epub 2002, Mar 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11914507 11914507]
+Structural plasticity in the eight-helix fold of a trematode haemoglobin., Milani M, Pesce A, Dewilde S, Ascenzi P, Moens L, Bolognesi M, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):719-22. Epub 2002, Mar 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11914507 11914507]
 [[Category: Paramphistomum epiclitum]]
 [[Category: Single protein]]
@@ Line 23: / Line 32: @@
 [[Category: hemoglobin]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:33:40 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:16:53 2008''