1k6d: Difference between revisions

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Revision as of 13:13, 20 March 2008

File:1k6d.gif

, resolution 1.90Å

Ligands:

Activity:

Acetate CoA-transferase, with EC number 2.8.3.8

Coordinates:

save as pdb, mmCIF, xml

CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT

OverviewOverview

The automation of protein structure determination is an essential component for high-throughput structural analysis in protein X-ray crystallography and is a key element in structural genomics. This highly challenging undertaking relies at present on the availability of high-quality native and derivatized protein crystals diffracting to high or moderate resolution, respectively. Obtaining such crystals often requires significant effort. The present study demonstrates that phases obtained at low resolution (>3.0 A) from crystals of SeMet-labeled protein can be successfully used for automated structure determination. The crystal structure of acetate CoA-transferase alpha-subunit was solved using 3.4 A multi-wavelength anomalous dispersion data collected from a crystal containing SeMet-substituted protein and 1.9 A data collected from a native protein crystal.

About this StructureAbout this Structure

1K6D is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

ReferenceReference

Autotracing of Escherichia coli acetate CoA-transferase alpha-subunit structure using 3.4 A MAD and 1.9 A native data., Korolev S, Koroleva O, Petterson K, Gu M, Collart F, Dementieva I, Joachimiak A, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2116-21. Epub 2002, Nov 23. PMID:12454473

Page seeded by OCA on Thu Mar 20 12:13:12 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1k6d.gif|left|200px]]<br /><applet load="1k6d" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1k6d.gif|left|200px]]
-caption="1k6d, resolution 1.90&Aring;" />
-'''CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT'''<br />
+ {{Structure
+|PDB= 1k6d |SIZE=350|CAPTION= <scene name='initialview01'>1k6d</scene>, resolution 1.90&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
+|ACTIVITY= [http://en.wikipedia.org/wiki/Acetate_CoA-transferase Acetate CoA-transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.3.8 2.8.3.8]
+|GENE=
+}}
+'''CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-K6D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Acetate_CoA-transferase Acetate CoA-transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.3.8 2.8.3.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6D OCA].
+K6D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6D OCA].
 ==Reference==
-Autotracing of Escherichia coli acetate CoA-transferase alpha-subunit structure using 3.4 A MAD and 1.9 A native data., Korolev S, Koroleva O, Petterson K, Gu M, Collart F, Dementieva I, Joachimiak A, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2116-21. Epub 2002, Nov 23. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12454473 12454473]
+Autotracing of Escherichia coli acetate CoA-transferase alpha-subunit structure using 3.4 A MAD and 1.9 A native data., Korolev S, Koroleva O, Petterson K, Gu M, Collart F, Dementieva I, Joachimiak A, Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2116-21. Epub 2002, Nov 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12454473 12454473]
 [[Category: Acetate CoA-transferase]]
 [[Category: Escherichia coli]]
@@ Line 23: / Line 32: @@
 [[Category: MG]]
 [[Category: mcsg]]
-[[Category: midwest center for structural genomics]]
+[[Category: midwest center for structural genomic]]
 [[Category: protein structure initiative]]
 [[Category: psi]]
-[[Category: structural genomics]]
+[[Category: structural genomic]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:30:36 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:13:12 2008''