2ux7

File:2ux7.gif

spinqualitylabelsall models 2ux7, resolution 1.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

PSEUDOAZURIN WITH ENGINEERED AMICYANIN LIGAND LOOP, REDUCED FORM, PH 7.5

OverviewOverview

Atomic resolution structures of the pseudoazurin (PAZ) variant into which, the shorter ligand-containing loop of amicyanin (AMI) is introduced have, been determined. The mutated loop adopts a different conformation in, PAZAMI than in AMI. The copper site structure is affected, with the major, influence being an increased axial interaction resulting in the shortest, Cu(II)-S(Met) bond observed for the cupredoxin family of electron-transfer, proteins. This is accompanied by a lengthening of the important Cu-S(Cys), bond and enhanced tetragonal distortion, consistent with the influence of, the PAZAMI loop contraction on the UV/vis spectrum. The change in active, site geometry is the major cause of the 50 mV decrease in reduction, potential. The copper site structure changes very little upon ... [(full description)]

About this StructureAbout this Structure

2UX7 is a [Single protein] structure of sequence from [Achromobacter cycloclastes] with CU, CL and GOL as [ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].

ReferenceReference

Influence of Loop Shortening on the Metal Binding Site of Cupredoxin Pseudoazurin(,)., Velarde M, Huber R, Yanagisawa S, Dennison C, Messerschmidt A, Biochemistry. 2007 Aug 9;. PMID:17685636

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