379d: Difference between revisions

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-[[Image:379d.png|left|200px]]
+==THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE==
+<StructureSection load='379d' size='340' side='right' caption='[[379d]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[379d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=379D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=379D FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=379d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=379d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=379d RCSB], [http://www.ebi.ac.uk/pdbsum/379d PDBsum]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We have captured an 8.7 A conformational change that takes place in the cleavage site of the hammerhead ribozyme during self-cleavage, using X-ray crystallography combined with physical and chemical trapping techniques. This rearrangement brings the hammerhead ribozyme from the ground state into a conformation that is poised to form the transition state geometry required for hammerhead RNA self-cleavage. Use of a 5'-C-methylated ribose adjacent to the cleavage site permits this ordinarily transient conformational change to be kinetically trapped and observed crystallographically after initiating the hammerhead ribozyme reaction in the crystal. Cleavage of the corresponding unmodified hammerhead ribozyme in the crystal under otherwise identical conditions is faster than in solution, indicating that we have indeed trapped a catalytically relevant intermediate form of this RNA enzyme.
-{{STRUCTURE_379d|  PDB=379d  |  SCENE=  }}
+The structural basis of hammerhead ribozyme self-cleavage.,Murray JB, Terwey DP, Maloney L, Karpeisky A, Usman N, Beigelman L, Scott WG Cell. 1998 Mar 6;92(5):665-73. PMID:9506521<ref>PMID:9506521</ref>
-===THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_9506521}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[379d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=379D OCA].
+</StructureSection>
-==See Also==
-*[[Ribozyme|Ribozyme]]
-==Reference==
-<ref group="xtra">PMID:009506521</ref><references group="xtra"/>
 [[Category: Beigelman, L.]]
 [[Category: Karpeisky, A.]]