1k4c: Difference between revisions
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'''Potassium Channel KcsA-Fab complex in high concentration of K+''' | {{Structure | ||
|PDB= 1k4c |SIZE=350|CAPTION= <scene name='initialview01'>1k4c</scene>, resolution 2.00Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=DGA:DIACYL+GLYCEROL'>DGA</scene> and <scene name='pdbligand=F09:NONAN-1-OL'>F09</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Potassium Channel KcsA-Fab complex in high concentration of K+''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1K4C is a [ | 1K4C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. The following page contains interesting information on the relation of 1K4C with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb38_1.html Potassium Channels]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K4C OCA]. | ||
==Reference== | ==Reference== | ||
Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution., Zhou Y, Morais-Cabral JH, Kaufman A, MacKinnon R, Nature. 2001 Nov 1;414(6859):43-8. PMID:[http:// | Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution., Zhou Y, Morais-Cabral JH, Kaufman A, MacKinnon R, Nature. 2001 Nov 1;414(6859):43-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11689936 11689936] | ||
[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Potassium Channels]] | [[Category: Potassium Channels]] | ||
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[[Category: protein-antibody fab complex]] | [[Category: protein-antibody fab complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:12:24 2008'' | ||
Revision as of 13:12, 20 March 2008
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Potassium Channel KcsA-Fab complex in high concentration of K+
OverviewOverview
Ion transport proteins must remove an ion's hydration shell to coordinate the ion selectively on the basis of its size and charge. To discover how the K+ channel solves this fundamental aspect of ion conduction, we solved the structure of the KcsA K+ channel in complex with a monoclonal Fab antibody fragment at 2.0 A resolution. Here we show how the K+ channel displaces water molecules around an ion at its extracellular entryway, and how it holds a K+ ion in a square antiprism of water molecules in a cavity near its intracellular entryway. Carbonyl oxygen atoms within the selectivity filter form a very similar square antiprism around each K+ binding site, as if to mimic the waters of hydration. The selectivity filter changes its ion coordination structure in low K+ solutions. This structural change is crucial to the operation of the selectivity filter in the cellular context, where the K+ ion concentration near the selectivity filter varies in response to channel gating.
About this StructureAbout this Structure
1K4C is a Single protein structure of sequence from Mus musculus and Streptomyces lividans. The following page contains interesting information on the relation of 1K4C with [Potassium Channels]. Full crystallographic information is available from OCA.
ReferenceReference
Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution., Zhou Y, Morais-Cabral JH, Kaufman A, MacKinnon R, Nature. 2001 Nov 1;414(6859):43-8. PMID:11689936
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