1k3s: Difference between revisions

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Type III Secretion Chaperone SigE

OverviewOverview

Several Gram-negative bacterial pathogens have evolved a type III secretion system to deliver virulence effector proteins directly into eukaryotic cells, a process essential for disease. This specialized secretion process requires customized chaperones specific for particular effector proteins. The crystal structures of the enterohemorrhagic Escherichia coli O157:H7 Tir-specific chaperone CesT and the Salmonella enterica SigD-specific chaperone SigE reveal a common overall fold and formation of homodimers. Site-directed mutagenesis suggests that variable, delocalized hydrophobic surfaces observed on the chaperone homodimers are responsible for specific binding to a particular effector protein. Isothermal titration calorimetry studies of Tir-CesT and enzymatic activity profiles of SigD-SigE indicate that the effector proteins are not globally unfolded in the presence of their cognate chaperones.

About this StructureAbout this Structure

1K3S is a Single protein structure of sequence from Salmonella enterica. Full crystallographic information is available from OCA.

ReferenceReference

Structural and biochemical characterization of the type III secretion chaperones CesT and SigE., Luo Y, Bertero MG, Frey EA, Pfuetzner RA, Wenk MR, Creagh L, Marcus SL, Lim D, Sicheri F, Kay C, Haynes C, Finlay BB, Strynadka NC, Nat Struct Biol. 2001 Dec;8(12):1031-6. PMID:11685226

Page seeded by OCA on Thu Mar 20 12:12:11 2008

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OCA

@@ Line 1: / Line 1: @@
-[[Image:1k3s.jpg|left|200px]]<br /><applet load="1k3s" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1k3s.jpg|left|200px]]
-caption="1k3s, resolution 1.9&Aring;" />
-'''Type III Secretion Chaperone SigE'''<br />
+ {{Structure
+|PDB= 1k3s |SIZE=350|CAPTION= <scene name='initialview01'>1k3s</scene>, resolution 1.9&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''Type III Secretion Chaperone SigE'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-K3S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica] with <scene name='pdbligand=PO4:'>PO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3S OCA].
+K3S is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica Salmonella enterica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3S OCA].
 ==Reference==
-Structural and biochemical characterization of the type III secretion chaperones CesT and SigE., Luo Y, Bertero MG, Frey EA, Pfuetzner RA, Wenk MR, Creagh L, Marcus SL, Lim D, Sicheri F, Kay C, Haynes C, Finlay BB, Strynadka NC, Nat Struct Biol. 2001 Dec;8(12):1031-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11685226 11685226]
+Structural and biochemical characterization of the type III secretion chaperones CesT and SigE., Luo Y, Bertero MG, Frey EA, Pfuetzner RA, Wenk MR, Creagh L, Marcus SL, Lim D, Sicheri F, Kay C, Haynes C, Finlay BB, Strynadka NC, Nat Struct Biol. 2001 Dec;8(12):1031-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11685226 11685226]
 [[Category: Salmonella enterica]]
 [[Category: Single protein]]
@@ Line 29: / Line 38: @@
 [[Category: type iii]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:29:51 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:12:11 2008''