1k3c: Difference between revisions
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'''Phosphoenolpyruvate carboxykinase in complex with ADP, AlF3 and Pyruvate''' | {{Structure | ||
|PDB= 1k3c |SIZE=350|CAPTION= <scene name='initialview01'>1k3c</scene>, resolution 2.0Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene> and <scene name='pdbligand=PYR:PYRUVIC ACID'>PYR</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Phosphoenolpyruvate_carboxykinase_(ATP) Phosphoenolpyruvate carboxykinase (ATP)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.49 4.1.1.49] | |||
|GENE= PCKA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''Phosphoenolpyruvate carboxykinase in complex with ADP, AlF3 and Pyruvate''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1K3C is a [ | 1K3C is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K3C OCA]. | ||
==Reference== | ==Reference== | ||
The phosphoryl-transfer mechanism of Escherichia coli phosphoenolpyruvate carboxykinase from the use of AlF(3)., Sudom AM, Prasad L, Goldie H, Delbaere LT, J Mol Biol. 2001 Nov 16;314(1):83-92. PMID:[http:// | The phosphoryl-transfer mechanism of Escherichia coli phosphoenolpyruvate carboxykinase from the use of AlF(3)., Sudom AM, Prasad L, Goldie H, Delbaere LT, J Mol Biol. 2001 Nov 16;314(1):83-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11724534 11724534] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Phosphoenolpyruvate carboxykinase (ATP)]] | [[Category: Phosphoenolpyruvate carboxykinase (ATP)]] | ||
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[[Category: p-loop]] | [[Category: p-loop]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:12:00 2008'' | ||
Revision as of 13:12, 20 March 2008
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| , resolution 2.0Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , and | ||||||
| Gene: | PCKA (Escherichia coli) | ||||||
| Activity: | Phosphoenolpyruvate carboxykinase (ATP), with EC number 4.1.1.49 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Phosphoenolpyruvate carboxykinase in complex with ADP, AlF3 and Pyruvate
OverviewOverview
The mechanism of reversible transfer of the gamma-phosphate group of ATP by Escherichia coli phosphoenolpyruvate carboxykinase (PCK) on to its substrate is of great interest. It is known that metallofluorides are accurate analogs of the transition state in the context of kinase mechanisms. Therefore, two complexes of PCK, one with AlF(3), Mg(2+) and ADP (complex I), the other with AlF(3), Mg(2+), ADP and pyruvate (complex II) were crystallized. The X-ray crystal structures of these two complexes were determined at 2.0 A resolution. The Al atom has trigonal bipyramidal geometry that mimics the transition state of phosphoryl transfer. The Al atom is at a distance of 2.8 A and 2.9 A from an oxygen atom of the beta-phosphoryl group of ADP in complex I and II, respectively. A water molecule in complex I and an oxygen atom of the pyruvate in complex II are located along the axis of the trigonal bipyramid on the side opposite to the beta-phosphoryl oxygen with respect to the equatorial plane, suggesting that the complexes are close mimics of the transition state. Along with the presence of positively charged species around the AlF(3) moiety, these results indicate that phosphoryl transfer occurs via a direct displacement mechanism with associative qualities.
About this StructureAbout this Structure
1K3C is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
The phosphoryl-transfer mechanism of Escherichia coli phosphoenolpyruvate carboxykinase from the use of AlF(3)., Sudom AM, Prasad L, Goldie H, Delbaere LT, J Mol Biol. 2001 Nov 16;314(1):83-92. PMID:11724534
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