1k03: Difference between revisions
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[[Image:1k03.jpg|left|200px]] | [[Image:1k03.jpg|left|200px]] | ||
'''Crystal Structure of Old Yellow Enzyme Mutant Gln114Asn Complexed with Para-hydroxy Benzaldehyde''' | {{Structure | ||
|PDB= 1k03 |SIZE=350|CAPTION= <scene name='initialview01'>1k03</scene>, resolution 2.7Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene> and <scene name='pdbligand=HBA:P-HYDROXYBENZALDEHYDE'>HBA</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] | |||
|GENE= | |||
}} | |||
'''Crystal Structure of Old Yellow Enzyme Mutant Gln114Asn Complexed with Para-hydroxy Benzaldehyde''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1K03 is a [ | 1K03 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K03 OCA]. | ||
==Reference== | ==Reference== | ||
The role of glutamine 114 in old yellow enzyme., Brown BJ, Hyun JW, Duvvuri S, Karplus PA, Massey V, J Biol Chem. 2002 Jan 18;277(3):2138-45. Epub 2001 Oct 19. PMID:[http:// | The role of glutamine 114 in old yellow enzyme., Brown BJ, Hyun JW, Duvvuri S, Karplus PA, Massey V, J Biol Chem. 2002 Jan 18;277(3):2138-45. Epub 2001 Oct 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11668181 11668181] | ||
[[Category: NADPH dehydrogenase]] | [[Category: NADPH dehydrogenase]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
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[[Category: HBA]] | [[Category: HBA]] | ||
[[Category: MG]] | [[Category: MG]] | ||
[[Category: beta-alpha | [[Category: beta-alpha barrel]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:10:49 2008'' | ||
Revision as of 13:10, 20 March 2008
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| , resolution 2.7Å | |||||||
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| Ligands: | , and | ||||||
| Activity: | NADPH dehydrogenase, with EC number 1.6.99.1 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Old Yellow Enzyme Mutant Gln114Asn Complexed with Para-hydroxy Benzaldehyde
OverviewOverview
Glutamine 114 of OYE1 is a well conserved residue in the active site of the Old Yellow Enzyme family. It forms hydrogen bonds to the O2 and N3 of the flavoprotein prosthetic group, FMN. Glutamine 114 was mutated to asparagine, introducing an R-group that is one methylene group shorter. The resultant enzyme was characterized to determine the effect of the mutation on the mechanistic behavior of the enzyme, and the crystal structure was solved to determine the effect of the mutation on the structure of the protein. The Q114N mutation results in little change in the protein structure, moving the amide group of residue 114 out of H-bonding distance, allowing repositioning of the FMN prosthetic group to form new interactions that replace the lost H-bonds. The mutation decreases the ability to bind ligands, as all dissociation constants for substituted phenols are larger than for the wild type enzyme. The rate constant for the reductive half-reaction with beta-NADPH is slightly greater, whereas that for the oxidative half-reaction with 2-cyclohexenone is smaller than for the wild type enzyme. Oxidation with molecular oxygen is biphasic and involves formation and reaction with O(2), a phenomenon that is more pronounced with this mutation than with wild type enzyme. When superoxide dismutase is added to the reaction, we observe a single-phase reaction typical of the wild type enzyme. Turnover reactions using beta-NADPH with 2-cyclohexenone and molecular oxygen were studied to further characterize the mutant enzyme.
About this StructureAbout this Structure
1K03 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
The role of glutamine 114 in old yellow enzyme., Brown BJ, Hyun JW, Duvvuri S, Karplus PA, Massey V, J Biol Chem. 2002 Jan 18;277(3):2138-45. Epub 2001 Oct 19. PMID:11668181
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