1jum: Difference between revisions

← Older edit Newer edit →

Revision as of 13:08, 20 March 2008

File:1jum.jpg

, resolution 2.98Å

Ligands:

and

Coordinates:

save as pdb, mmCIF, xml

Crystal structure of the multidrug binding transcriptional repressor QacR bound to the natural drug berberine

OverviewOverview

The Staphylococcus aureus multidrug binding protein QacR represses transcription of the qacA multidrug transporter gene and is induced by structurally diverse cationic lipophilic drugs. Here, we report the crystal structures of six QacR-drug complexes. Compared to the DNA bound structure, drug binding elicits a coil-to-helix transition that causes induction and creates an expansive multidrug-binding pocket, containing four glutamates and multiple aromatic and polar residues. These structures indicate the presence of separate but linked drug-binding sites within a single protein. This multisite drug-binding mechanism is consonant with studies on multidrug resistance transporters.

About this StructureAbout this Structure

1JUM is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.

ReferenceReference

Structural mechanisms of QacR induction and multidrug recognition., Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG, Science. 2001 Dec 7;294(5549):2158-63. PMID:11739955

Page seeded by OCA on Thu Mar 20 12:08:44 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1jum.jpg|left|200px]]<br /><applet load="1jum" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1jum.jpg|left|200px]]
-caption="1jum, resolution 2.98&Aring;" />
-'''Crystal structure of the multidrug binding transcriptional repressor QacR bound to the natural drug berberine'''<br />
+ {{Structure
+|PDB= 1jum |SIZE=350|CAPTION= <scene name='initialview01'>1jum</scene>, resolution 2.98&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=BER:BERBERINE'>BER</scene>
+|ACTIVITY=
+|GENE=
+}}
+'''Crystal structure of the multidrug binding transcriptional repressor QacR bound to the natural drug berberine'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-JUM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=BER:'>BER</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JUM OCA].
+JUM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JUM OCA].
 ==Reference==
-Structural mechanisms of QacR induction and multidrug recognition., Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG, Science. 2001 Dec 7;294(5549):2158-63. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11739955 11739955]
+Structural mechanisms of QacR induction and multidrug recognition., Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG, Science. 2001 Dec 7;294(5549):2158-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11739955 11739955]
 [[Category: Single protein]]
 [[Category: Staphylococcus aureus]]
@@ Line 29: / Line 38: @@
 [[Category: s. aureus]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:26:54 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:08:44 2008''