1jt0: Difference between revisions
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[[Image:1jt0.gif|left|200px]] | [[Image:1jt0.gif|left|200px]] | ||
'''Crystal structure of a cooperative QacR-DNA complex''' | {{Structure | ||
|PDB= 1jt0 |SIZE=350|CAPTION= <scene name='initialview01'>1jt0</scene>, resolution 2.90Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of a cooperative QacR-DNA complex''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1JT0 is a [ | 1JT0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JT0 OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis for cooperative DNA binding by two dimers of the multidrug-binding protein QacR., Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG, EMBO J. 2002 Mar 1;21(5):1210-8. PMID:[http:// | Structural basis for cooperative DNA binding by two dimers of the multidrug-binding protein QacR., Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG, EMBO J. 2002 Mar 1;21(5):1210-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11867549 11867549] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
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[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: cooperative dna binding]] | [[Category: cooperative dna binding]] | ||
[[Category: dimer of | [[Category: dimer of dimer]] | ||
[[Category: multidrug binding protein]] | [[Category: multidrug binding protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:08:01 2008'' | ||
Revision as of 13:08, 20 March 2008
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| , resolution 2.90Å | |||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of a cooperative QacR-DNA complex
OverviewOverview
The Staphylococcus aureus multidrug-binding protein QacR represses transcription of the qacA multidrug transporter gene and is induced by multiple structurally dissimilar drugs. QacR is a member of the TetR/CamR family of transcriptional regulators, which share highly homologous N-terminal DNA-binding domains connected to seemingly non-homologous ligand-binding domains. Unlike other TetR members, which bind approximately 15 bp operators, QacR recognizes an unusually long 28 bp operator, IR1, which it appears to bind cooperatively. To elucidate the DNA-binding mechanism of QacR, we determined the 2.90 A resolution crystal structure of a QacR-IR1 complex. Strikingly, our data reveal that the DNA recognition mode of QacR is distinct from TetR and involves the binding of a pair of QacR dimers. In this unique binding mode, recognition at each IR1 half-site is mediated by a complement of DNA contacts made by two helix-turn-helix motifs. The inferred cooperativity does not arise from cross-dimer protein-protein contacts, but from the global undertwisting and major groove widening elicited by the binding of two QacR dimers.
About this StructureAbout this Structure
1JT0 is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for cooperative DNA binding by two dimers of the multidrug-binding protein QacR., Schumacher MA, Miller MC, Grkovic S, Brown MH, Skurray RA, Brennan RG, EMBO J. 2002 Mar 1;21(5):1210-8. PMID:11867549
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