1jra: Difference between revisions

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Revision as of 13:07, 20 March 2008

File:1jra.jpg

, resolution 2.0Å

Ligands:

Gene:

ERV2 (Saccharomyces cerevisiae)

Coordinates:

save as pdb, mmCIF, xml

Crystal Structure of Erv2p

OverviewOverview

Erv2p is an FAD-dependent sulfhydryl oxidase that can promote disulfide bond formation during protein biosynthesis in the yeast endoplasmic reticulum. The structure of Erv2p, determined by X-ray crystallography to 1.5 A resolution, reveals a helix-rich dimer with no global resemblance to other known FAD-binding proteins or thiol oxidoreductases. Two pairs of cysteine residues are required for Erv2p activity. The first (Cys-Gly-Glu-Cys) is adjacent to the isoalloxazine ring of the FAD. The second (Cys-Gly-Cys) is part of a flexible C-terminal segment that can swing into the vicinity of the first cysteine pair in the opposite subunit of the dimer and may shuttle electrons between substrate protein dithiols and the FAD-proximal disulfide.

About this StructureAbout this Structure

1JRA is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

ReferenceReference

A new FAD-binding fold and intersubunit disulfide shuttle in the thiol oxidase Erv2p., Gross E, Sevier CS, Vala A, Kaiser CA, Fass D, Nat Struct Biol. 2002 Jan;9(1):61-7. PMID:11740506

Page seeded by OCA on Thu Mar 20 12:07:22 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-[[Image:1jra.jpg|left|200px]]<br /><applet load="1jra" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1jra.jpg|left|200px]]
-caption="1jra, resolution 2.0&Aring;" />
-'''Crystal Structure of Erv2p'''<br />
+ {{Structure
+|PDB= 1jra |SIZE=350|CAPTION= <scene name='initialview01'>1jra</scene>, resolution 2.0&Aring;
+|SITE=
+|LIGAND= <scene name='pdbligand=FAD:FLAVIN-ADENINE DINUCLEOTIDE'>FAD</scene>
+|ACTIVITY=
+|GENE= ERV2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])
+}}
+'''Crystal Structure of Erv2p'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-JRA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with <scene name='pdbligand=FAD:'>FAD</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JRA OCA].
+JRA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JRA OCA].
 ==Reference==
-A new FAD-binding fold and intersubunit disulfide shuttle in the thiol oxidase Erv2p., Gross E, Sevier CS, Vala A, Kaiser CA, Fass D, Nat Struct Biol. 2002 Jan;9(1):61-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11740506 11740506]
+A new FAD-binding fold and intersubunit disulfide shuttle in the thiol oxidase Erv2p., Gross E, Sevier CS, Vala A, Kaiser CA, Fass D, Nat Struct Biol. 2002 Jan;9(1):61-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11740506 11740506]
 [[Category: Saccharomyces cerevisiae]]
 [[Category: Single protein]]
@@ Line 24: / Line 33: @@
 [[Category: sulfhydryl oxidase]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:25:50 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:07:22 2008''