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''' | ==Crystal structure of the PCE reductive dehalogenase from S. multivorans in complex with trichloroethene== | ||
<StructureSection load='4ur0' size='340' side='right' caption='[[4ur0]], [[Resolution|resolution]] 1.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4ur0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfurospirillum_multivorans Sulfurospirillum multivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UR0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4UR0 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=BVQ:NORPSEUDO-B12'>BVQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=TCV:1,1,2-TRICHLOROETHENE'>TCV</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uqu|4uqu]], [[4ur1|4ur1]], [[4ur2|4ur2]], [[4ur3|4ur3]]</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Tetrachloroethene_reductive_dehalogenase Tetrachloroethene reductive dehalogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.97.1.8 1.97.1.8] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ur0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ur0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ur0 RCSB], [http://www.ebi.ac.uk/pdbsum/4ur0 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Organohalide-respiring microorganisms can use a variety of persistent pollutants including trichloroethene (TCE) as terminal electron acceptors. The final two-electron transfer step in organohalide respiration is catalyzed by reductive dehalogenases. Here we report the x-ray crystal structure of PceA, an archetypal dehalogenase from Sulfurospirillum multivorans, as well as structures of PceA in complex with TCE and product analogs. The active site harbors a deeply buried norpseudo-B12 cofactor within a nitroreductase fold, also found in a mammalian B12 chaperone. The structures of PceA reveal how a cobalamin supports a reductive haloelimination exploiting a conserved B12-binding scaffold capped by a highly variable substrate-capturing region. | |||
Structural basis for organohalide respiration.,Bommer M, Kunze C, Fesseler J, Schubert T, Diekert G, Dobbek H Science. 2014 Oct 2. pii: 1258118. PMID:25278505<ref>PMID:25278505</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Sulfurospirillum multivorans]] | |||
[[Category: Tetrachloroethene reductive dehalogenase]] | |||
[[Category: Bommer, M.]] | |||
[[Category: Diekert, G.]] | |||
[[Category: Dobbek, H.]] | |||
[[Category: Fesseler, J.]] | |||
[[Category: Kunze, C.]] | |||
[[Category: Schubert, T.]] | |||
[[Category: Oxidoreductase]] | |||
Revision as of 13:44, 20 October 2014
Crystal structure of the PCE reductive dehalogenase from S. multivorans in complex with trichloroetheneCrystal structure of the PCE reductive dehalogenase from S. multivorans in complex with trichloroethene
Structural highlights
Publication Abstract from PubMedOrganohalide-respiring microorganisms can use a variety of persistent pollutants including trichloroethene (TCE) as terminal electron acceptors. The final two-electron transfer step in organohalide respiration is catalyzed by reductive dehalogenases. Here we report the x-ray crystal structure of PceA, an archetypal dehalogenase from Sulfurospirillum multivorans, as well as structures of PceA in complex with TCE and product analogs. The active site harbors a deeply buried norpseudo-B12 cofactor within a nitroreductase fold, also found in a mammalian B12 chaperone. The structures of PceA reveal how a cobalamin supports a reductive haloelimination exploiting a conserved B12-binding scaffold capped by a highly variable substrate-capturing region. Structural basis for organohalide respiration.,Bommer M, Kunze C, Fesseler J, Schubert T, Diekert G, Dobbek H Science. 2014 Oct 2. pii: 1258118. PMID:25278505[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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