1jnv: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1jnv.jpg|left|200px]] | [[Image:1jnv.jpg|left|200px]] | ||
'''The Conformation of the Epsilon and Gamma Subunits within the E. coli F1 ATPase''' | {{Structure | ||
|PDB= 1jnv |SIZE=350|CAPTION= <scene name='initialview01'>1jnv</scene>, resolution 4.4Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/H(+)-transporting_two-sector_ATPase H(+)-transporting two-sector ATPase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.3.14 3.6.3.14] | |||
|GENE= atpA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), atpD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), atpC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), atpG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''The Conformation of the Epsilon and Gamma Subunits within the E. coli F1 ATPase''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1JNV is a [ | 1JNV is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JNV OCA]. | ||
==Reference== | ==Reference== | ||
The conformation of the epsilon- and gamma-subunits within the Escherichia coli F(1) ATPase., Hausrath AC, Capaldi RA, Matthews BW, J Biol Chem. 2001 Dec 14;276(50):47227-32. Epub 2001 Oct 3. PMID:[http:// | The conformation of the epsilon- and gamma-subunits within the Escherichia coli F(1) ATPase., Hausrath AC, Capaldi RA, Matthews BW, J Biol Chem. 2001 Dec 14;276(50):47227-32. Epub 2001 Oct 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11585832 11585832] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: H(+)-transporting two-sector ATPase]] | [[Category: H(+)-transporting two-sector ATPase]] | ||
| Line 18: | Line 27: | ||
[[Category: Matthews, B W.]] | [[Category: Matthews, B W.]] | ||
[[Category: atp synthase]] | [[Category: atp synthase]] | ||
[[Category: | [[Category: bioenergetic]] | ||
[[Category: f1 atpase]] | [[Category: f1 atpase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:05:56 2008'' | ||
Revision as of 13:05, 20 March 2008
| |||||||
| , resolution 4.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | atpA (Escherichia coli), atpD (Escherichia coli), atpC (Escherichia coli), atpG (Escherichia coli) | ||||||
| Activity: | H(+)-transporting two-sector ATPase, with EC number 3.6.3.14 | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The Conformation of the Epsilon and Gamma Subunits within the E. coli F1 ATPase
OverviewOverview
F(1) is the water-soluble portion of the ubiquitous F(1)F(0) ATP synthase. Its structure includes three alpha- and three beta-subunits, arranged as a hexameric disc, plus a gamma-subunit that penetrates the center of the disc akin to an axle. Recently Hausrath et al. (Hausrath, A. C., Gruber, G., Matthews, B. W., and Capaldi, R. A. (1999) Proc. Natl. Acad. Sci. U. S. A. 96, 13697-13702) obtained an electron density map of E. coli F(1) at 4.4-A resolution in which the coiled-coil alpha-helices of the gamma-subunit could be seen to extend 45 A from the base of the alpha(3)beta(3) hexamer. Subsequently the structure of a truncated form of the E. coli gamma-subunit in complex with epsilon has been described (Rodgers, A. J. W., and Wilce, M. C. J. (2000) Nat. Struct. Biol. 7, 1051-1054). In the present study the 4.4-A resolution electron density map of E. coli F(1) is re-evaluated in light of the newly available data on the gamma- and epsilon-subunits. It is shown that the map of the F(1) complex is consistent with the structure of the isolated subunits. When E. coli F(1) is compared with that from beef heart, the structures of the E. coli gamma- and epsilon-subunits are seen to be generally similar to their counterparts in the bovine enzyme but to undergo major shifts in position. In particular, the two long, coiled-coil alpha-helices that lie along the axis of F(1) both unwind and rotate. Also the epsilon-subunit rotates around the axis by 81 degrees and undergoes a net translation of about 23 A. It is argued that these large-scale changes in conformation reflect distinct functional states that occur during the rotation of the gamma-subunit within the alpha(3)beta(3) hexamer.
About this StructureAbout this Structure
1JNV is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
ReferenceReference
The conformation of the epsilon- and gamma-subunits within the Escherichia coli F(1) ATPase., Hausrath AC, Capaldi RA, Matthews BW, J Biol Chem. 2001 Dec 14;276(50):47227-32. Epub 2001 Oct 3. PMID:11585832
Page seeded by OCA on Thu Mar 20 12:05:56 2008