1jj6: Difference between revisions
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[[Image:1jj6.gif|left|200px]] | [[Image:1jj6.gif|left|200px]] | ||
'''Testing the Water-Mediated Hin Recombinase DNA Recognition by Systematic Mutations.''' | {{Structure | ||
|PDB= 1jj6 |SIZE=350|CAPTION= <scene name='initialview01'>1jj6</scene>, resolution 2.30Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Testing the Water-Mediated Hin Recombinase DNA Recognition by Systematic Mutations.''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1JJ6 is a [ | 1JJ6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJ6 OCA]. | ||
==Reference== | ==Reference== | ||
Testing water-mediated DNA recognition by the Hin recombinase., Chiu TK, Sohn C, Dickerson RE, Johnson RC, EMBO J. 2002 Feb 15;21(4):801-14. PMID:[http:// | Testing water-mediated DNA recognition by the Hin recombinase., Chiu TK, Sohn C, Dickerson RE, Johnson RC, EMBO J. 2002 Feb 15;21(4):801-14. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11847127 11847127] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Chiu, T K.]] | [[Category: Chiu, T K.]] | ||
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[[Category: water-mediated recognition]] | [[Category: water-mediated recognition]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:04:07 2008'' | ||
Revision as of 13:04, 20 March 2008
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Testing the Water-Mediated Hin Recombinase DNA Recognition by Systematic Mutations.
OverviewOverview
The Hin recombinase specifically recognizes its DNA-binding site by means of both major and minor groove interactions. A previous X-ray structure, together with new structures of the Hin DNA-binding domain bound to a recombination half-site that were solved as part of the present study, have revealed that two ordered water molecules are present within the major groove interface. In this report, we test the importance of these waters directly by X-ray crystal structure analysis of complexes with four mutant DNA sequences. These structures, combined with their Hin-binding properties, provide strong support for the critical importance of one of the intermediate waters. A lesser but demonstrable role is ascribed to the second water molecule. The mutant structures also illustrate the prominent roles of thymine methyls both in stabilizing intermediate waters and in interfering with water or amino acid side chain interactions with DNA.
About this StructureAbout this Structure
1JJ6 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
ReferenceReference
Testing water-mediated DNA recognition by the Hin recombinase., Chiu TK, Sohn C, Dickerson RE, Johnson RC, EMBO J. 2002 Feb 15;21(4):801-14. PMID:11847127
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