1jgp: Difference between revisions

Revision as of 13:03, 20 March 2008

, resolution 7.0Å

Coordinates:

The Path of Messenger RNA Through the Ribosome. THIS FILE, 1JGP, CONTAINS THE 30S RIBOSOME SUBUNIT, THREE TRNA, AND MRNA MOLECULES. 50S RIBOSOME SUBUNIT IS IN THE FILE 1GIY

OverviewOverview

Using X-ray crystallography, we have directly observed the path of mRNA in the 70S ribosome in Fourier difference maps at 7 A resolution. About 30 nucleotides of the mRNA are wrapped in a groove that encircles the neck of the 30S subunit. The Shine-Dalgarno helix is bound in a large cleft between the head and the back of the platform. At the interface, only about eight nucleotides (-1 to +7), centered on the junction between the A and P codons, are exposed, and bond almost exclusively to 16S rRNA. The mRNA enters the ribosome around position +13 to +15, the location of downstream pseudoknots that stimulate -1 translational frame shifting.

About this StructureAbout this Structure

1JGP is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

ReferenceReference

The path of messenger RNA through the ribosome., Yusupova GZ, Yusupov MM, Cate JH, Noller HF, Cell. 2001 Jul 27;106(2):233-41. PMID:11511350

Page seeded by OCA on Thu Mar 20 12:03:08 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-[[Image:1jgp.gif|left|200px]]<br /><applet load="1jgp" size="350" color="white" frame="true" align="right" spinBox="true"
+[[Image:1jgp.gif|left|200px]]
-caption="1jgp, resolution 7.0&Aring;" />
-'''The Path of Messenger RNA Through the Ribosome. THIS FILE, 1JGP, CONTAINS THE 30S RIBOSOME SUBUNIT, THREE TRNA, AND MRNA MOLECULES. 50S RIBOSOME SUBUNIT IS IN THE FILE 1GIY'''<br />
+ {{Structure
+|PDB= 1jgp |SIZE=350|CAPTION= <scene name='initialview01'>1jgp</scene>, resolution 7.0&Aring;
+|SITE=
+|LIGAND=
+|ACTIVITY=
+|GENE=
+}}
+'''The Path of Messenger RNA Through the Ribosome. THIS FILE, 1JGP, CONTAINS THE 30S RIBOSOME SUBUNIT, THREE TRNA, AND MRNA MOLECULES. 50S RIBOSOME SUBUNIT IS IN THE FILE 1GIY'''
 ==Overview==
@@ Line 7: / Line 16: @@
 ==About this Structure==
-JGP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JGP OCA].
+JGP is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JGP OCA].
 ==Reference==
-The path of messenger RNA through the ribosome., Yusupova GZ, Yusupov MM, Cate JH, Noller HF, Cell. 2001 Jul 27;106(2):233-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11511350 11511350]
+The path of messenger RNA through the ribosome., Yusupova GZ, Yusupov MM, Cate JH, Noller HF, Cell. 2001 Jul 27;106(2):233-41. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11511350 11511350]
 [[Category: Protein complex]]
 [[Category: Thermus thermophilus]]
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 [[Category: ribosome assembly]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 13:22:28 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:03:08 2008''