1saq: Difference between revisions
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[[ | ==Crystal Structure of the RNA octamer GIC(GA)GCC== | ||
<StructureSection load='1saq' size='340' side='right' caption='[[1saq]], [[Resolution|resolution]] 2.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1saq]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SAQ FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=I:INOSINIC+ACID'>I</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1sa9|1sa9]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1saq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1saq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1saq RCSB], [http://www.ebi.ac.uk/pdbsum/1saq PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The crystal structures of two RNA octamers, 5'-GGC(GA)GCC-3' and 5'-GIC(GA)GCC-3', have been determined from X-ray diffraction data to 2.8 and 2.7 A resolution, respectively. The RNA octamers crystallize in isomorphous unit cells containing two mispairs arranged in a self-complementary manner and one single strand in the asymmetric unit. The single strand pairs with another single strand related by crystallographic symmetry to form a third unique double helix. Tandem non-Watson-Crick G.A/A.G base pairs of the sheared type comprise an internal loop in the middle of each duplex. The NMR structure of this octameric RNA sequence is also known, allowing comparison of the variation between the six crystallographic duplexes and the solution structure. In the symmetric duplex of the octamer containing inosine, the sheared G.A pairs incorporate a bound water molecule. This duplex also binds one water molecule per strand in the minor groove adjacent to the G.A pairs. | |||
Structures of two RNA octamers containing tandem G.A base pairs.,Jang SB, Baeyens K, Jeong MS, SantaLucia J Jr, Turner D, Holbrook SR Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):829-35. Epub 2004, Apr 21. PMID:15103128<ref>PMID:15103128</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Baeyens, K.]] | [[Category: Baeyens, K.]] | ||
[[Category: Holbrook, S R.]] | [[Category: Holbrook, S R.]] | ||
Revision as of 11:25, 8 October 2014
Crystal Structure of the RNA octamer GIC(GA)GCCCrystal Structure of the RNA octamer GIC(GA)GCC
Structural highlights
Publication Abstract from PubMedThe crystal structures of two RNA octamers, 5'-GGC(GA)GCC-3' and 5'-GIC(GA)GCC-3', have been determined from X-ray diffraction data to 2.8 and 2.7 A resolution, respectively. The RNA octamers crystallize in isomorphous unit cells containing two mispairs arranged in a self-complementary manner and one single strand in the asymmetric unit. The single strand pairs with another single strand related by crystallographic symmetry to form a third unique double helix. Tandem non-Watson-Crick G.A/A.G base pairs of the sheared type comprise an internal loop in the middle of each duplex. The NMR structure of this octameric RNA sequence is also known, allowing comparison of the variation between the six crystallographic duplexes and the solution structure. In the symmetric duplex of the octamer containing inosine, the sheared G.A pairs incorporate a bound water molecule. This duplex also binds one water molecule per strand in the minor groove adjacent to the G.A pairs. Structures of two RNA octamers containing tandem G.A base pairs.,Jang SB, Baeyens K, Jeong MS, SantaLucia J Jr, Turner D, Holbrook SR Acta Crystallogr D Biol Crystallogr. 2004 May;60(Pt 5):829-35. Epub 2004, Apr 21. PMID:15103128[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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